Ascorbic acid deficiency leads to increased grain chalkiness in transgenic rice for suppressed of L-GalLDH

Ascorbic acid deficiency leads to increased grain chalkiness in transgenic rice for suppressed of L-GalLDH

Copyright © 2017 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 211(2017) vom: 01. Apr., Seite 13-26
1. Verfasser: Yu, Le (VerfasserIn)
Weitere Verfasser: Liu, Yonghai, Lu, Lina, Zhang, Qilei, Chen, Yezheng, Zhou, Liping, Chen, Hua, Peng, Changlian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Antioxidant Ascorbic acid Grain chalkiness Rice Starch l-Galactono-14-lactone dehydrogenase Antioxidants Free Radical Scavengers RNA, Messenger mehr... Chlorophyll 1406-65-1 Hydroxyl Radical 3352-57-6 Malondialdehyde 4Y8F71G49Q 9005-25-8 Hydrogen Peroxide BBX060AN9V Oxidoreductases Acting on CH-CH Group Donors EC 1.3.- galactonolactone dehydrogenase EC 1.3.2.3 Ribulose-Bisphosphate Carboxylase EC 4.1.1.39 Ascorbic Acid PQ6CK8PD0R
Beschreibung
Zusammenfassung:Copyright © 2017 Elsevier GmbH. All rights reserved.
The grain chalkiness of rice (Oryza sativa L.), which determines the rice quality and price, is a major concern in rice breeding. Reactive oxygen species (ROS) plays a critical role in regulating rice endosperm chalkiness. Ascorbic acid (Asc) is a major plant antioxidant, which strictly regulates the levels of ROS. l-galactono-1, 4-lactone dehydrogenase (L-GalLDH, EC 1.3.2.3) is an enzyme that catalyzes the last step of Asc biosynthesis in higher plants. Here we show that the L-GalLDH-suppressed transgenic rice, GI-1 and GI-2, which have constitutively low (between 30% and 50%) leaf and grain Asc content compared with the wild-type (WT), exhibit significantly increased grain chalkiness. Further examination showed that the deficiency of Asc resulted in a higher lipid peroxidation and H2O2 content, accompanied by a lower hydroxyl radical scavenging rate, total antioxidant capacity and photosynthetic ability. In addition, changes of the enzyme activities and gene transcript abundances related to starch synthesis were also observed in GI-1 and GI-2 grains. The results we presented here suggest a close correlation between Asc deficiency and grain chalkiness in the L-GalLDH-suppressed transgenics. Asc deficiency leads to the accumulation of H2O2, affecting antioxidant capacity and photosynthetic function, changing enzyme activities and gene transcript abundances related to starch synthesis, finally leading to the increased grain chalkiness
Beschreibung:Date Completed 26.06.2017
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2016.11.017