Distinct regions of the Phytophthora essential effector Avh238 determine its function in cell death activation and plant immunity suppression

Distinct regions of the Phytophthora essential effector Avh238 determine its function in cell death activation and plant immunity suppression

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 214(2017), 1 vom: 23. Apr., Seite 361-375
1. Verfasser: Yang, Bo (VerfasserIn)
Weitere Verfasser: Wang, Qunqing, Jing, Maofeng, Guo, Baodian, Wu, Jiawei, Wang, Haonan, Wang, Yang, Lin, Long, Wang, Yan, Ye, Wenwu, Dong, Suomeng, Wang, Yuanchao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Phytophthora sojae RXLR effector cell death immunosuppression localization virulence Proteins
Beschreibung
Zusammenfassung:© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
Phytophthora pathogens secrete effectors to manipulate host innate immunity, thus facilitating infection. Among the RXLR effectors highly induced during Phytophthora sojae infection, Avh238 not only contributes to pathogen virulence but also triggers plant cell death. However, the detailed molecular basis of Avh238 functions remains largely unknown. We mapped the regions responsible for Avh238 functions in pathogen virulence and plant cell death induction using a strategy that combines investigation of natural variation and large-scale mutagenesis assays. The correlation between cellular localization and Avh238 functions was also evaluated. We found that the 79th residue (histidine or leucine) of Avh238 determined its cell death-inducing activity, and that the 53 amino acids in its C-terminal region are responsible for promoting Phytophthora infection. Transient expression of Avh238 in Nicotiana benthamiana revealed that nuclear localization is essential for triggering cell death, while Avh238-mediated suppression of INF1-triggered cell death requires cytoplasmic localization. Our results demonstrate that a representative example of an essential Phytophthora RXLR effector can evolve to escape recognition by the host by mutating one nucleotide site, and can also retain plant immunosuppressive activity to enhance pathogen virulence in planta
Beschreibung:Date Completed 16.02.2018
Date Revised 09.04.2022
published: Print-Electronic
CommentIn: New Phytol. 2017 Apr;214(1):8-10. - PMID 28239893
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.14430