Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during in vitro shoot regeneration of Nicotiana tabacum callus : Implications for bioprocess routes to plant-made biopharmaceuticals

Transplastomic plants are capable of high-yield production of recombinant biopharmaceutical proteins. Plant tissue culture combines advantages of agricultural cultivation with the bioprocess consistency associated with suspension culture. Overexpression of recombinant proteins through regeneration o...

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Veröffentlicht in:	Biochemical engineering journal. - 1998. - 117(2017), Pt B vom: 15. Jan., Seite 73-81
1. Verfasser:	Barretto, Sherwin S (VerfasserIn)
Weitere Verfasser:	Michoux, Franck, Hellgardt, Klaus, Nixon, Peter J
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	Biochemical engineering journal
Schlagworte:	Journal Article Biopharmaceutical CIM, callus induction medium Hydrodynamics MS medium, Murashige & Skoog medium Pneumatic energy dissipation RITA®, recipient for automated temporary immersion (translated from French) SDS-PAGE, sodium dodecyl sulphate polyacrylamide gel electrophoresis TF, triphenylformazan TIB, temporary immersion bioreactor mehr... TSP, total soluble protein TTC, 2,3,5-triphenyltetrazolium chloride Temporary immersion culture TetC, fragment C of tetanus toxin Transplastomic protein in vitro organogenesis kDa, kiloDalton