Divergent cytosine DNA methylation patterns in single-cell, soybean root hairs

Divergent cytosine DNA methylation patterns in single-cell, soybean root hairs

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 214(2017), 2 vom: 18. Apr., Seite 808-819
1. Verfasser: Hossain, Md Shakhawat (VerfasserIn)
Weitere Verfasser: Kawakatsu, Taiji, Kim, Kyung Do, Zhang, Ning, Nguyen, Cuong T, Khan, Saad M, Batek, Josef M, Joshi, Trupti, Schmutz, Jeremy, Grimwood, Jane, Schmitz, Robert J, Xu, Dong, Jackson, Scott A, Ecker, Joseph R, Stacey, Gary
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article DNA methylation bisulfite sequencing differentially methylated regions (DMRs) epigenetics heat stress root hairs single cell stripped roots DNA Transposable Elements mehr... Cytosine 8J337D1HZY
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520 |a Chromatin modifications, such as cytosine methylation of DNA, play a significant role in mediating gene expression in plants, which affects growth, development, and cell differentiation. As root hairs are single-cell extensions of the root epidermis and the primary organs for water uptake and nutrients, we sought to use root hairs as a single-cell model system to measure the impact of environmental stress. We measured changes in cytosine DNA methylation in single-cell root hairs as compared with multicellular stripped roots, as well as in response to heat stress. Differentially methylated regions (DMRs) in each methylation context showed very distinct methylation patterns between cell types and in response to heat stress. Intriguingly, at normal temperature, root hairs were more hypermethylated than were stripped roots. However, in response to heat stress, both root hairs and stripped roots showed hypomethylation in each context, especially in the CHH context. Moreover, expression analysis of mRNA from similar tissues and treatments identified some associations between DMRs, genes and transposons. Taken together, the data indicate that changes in DNA methylation are directly or indirectly associated with expression of genes and transposons within the context of either specific tissues/cells or stress (heat) 
650 4 |a Journal Article 
650 4 |a DNA methylation 
650 4 |a bisulfite sequencing 
650 4 |a differentially methylated regions (DMRs) 
650 4 |a epigenetics 
650 4 |a heat stress 
650 4 |a root hairs 
650 4 |a single cell 
650 4 |a stripped roots 
650 7 |a DNA Transposable Elements  |2 NLM 
650 7 |a Cytosine  |2 NLM 
650 7 |a 8J337D1HZY  |2 NLM 
700 1 |a Kawakatsu, Taiji  |e verfasserin  |4 aut 
700 1 |a Kim, Kyung Do  |e verfasserin  |4 aut 
700 1 |a Zhang, Ning  |e verfasserin  |4 aut 
700 1 |a Nguyen, Cuong T  |e verfasserin  |4 aut 
700 1 |a Khan, Saad M  |e verfasserin  |4 aut 
700 1 |a Batek, Josef M  |e verfasserin  |4 aut 
700 1 |a Joshi, Trupti  |e verfasserin  |4 aut 
700 1 |a Schmutz, Jeremy  |e verfasserin  |4 aut 
700 1 |a Grimwood, Jane  |e verfasserin  |4 aut 
700 1 |a Schmitz, Robert J  |e verfasserin  |4 aut 
700 1 |a Xu, Dong  |e verfasserin  |4 aut 
700 1 |a Jackson, Scott A  |e verfasserin  |4 aut 
700 1 |a Ecker, Joseph R  |e verfasserin  |4 aut 
700 1 |a Stacey, Gary  |e verfasserin  |4 aut 
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773 1 8 |g volume:214  |g year:2017  |g number:2  |g day:18  |g month:04  |g pages:808-819 
856 4 0 |u http://dx.doi.org/10.1111/nph.14421  |3 Volltext 
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