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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2016.12.005
|2 doi
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|a pubmed25n0892.xml
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|a (DE-627)NLM267712898
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|a (NLM)28056387
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|a (PII)S0176-1617(16)30283-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Uhlíková, Hana
|e verfasserin
|4 aut
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|a MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 17.05.2017
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier GmbH. All rights reserved.
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|a Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins
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|a Journal Article
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|a ATPase
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|a Cryptogein
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|a Glutamine synthetase
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|a NADP-malic enzyme
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|a Rab8
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|a Resistance
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|a Fungal Proteins
|2 NLM
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|a Membrane Proteins
|2 NLM
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|a Pathogen-Associated Molecular Pattern Molecules
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Trypsin
|2 NLM
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|a EC 3.4.21.4
|2 NLM
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| 700 |
1 |
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|a Solanský, Martin
|e verfasserin
|4 aut
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1 |
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|a Hrdinová, Vendula
|e verfasserin
|4 aut
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| 700 |
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|a Šedo, Ondrej
|e verfasserin
|4 aut
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|a Kašparovský, Tomáš
|e verfasserin
|4 aut
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|a Hejátko, Jan
|e verfasserin
|4 aut
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| 700 |
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|a Lochman, Jan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 210(2017) vom: 01. März, Seite 51-57
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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|g volume:210
|g year:2017
|g day:01
|g month:03
|g pages:51-57
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|u http://dx.doi.org/10.1016/j.jplph.2016.12.005
|3 Volltext
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