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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b03778
|2 doi
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|a pubmed24n0892.xml
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|a (DE-627)NLM267642954
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|a (NLM)28045529
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Riabtseva, Anna
|e verfasserin
|4 aut
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| 245 |
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|a Polyelectrolyte pH-Responsive Protein-Containing Nanoparticles
|b The Physicochemical Supramolecular Approach
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.09.2018
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|a Date Revised 02.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We report on the physicochemical properties and self-assembly behavior of novel efficient pH-sensitive nanocontainers based on the Food and Drug Administration-approved anionic polymer Eudragit L100-55 (poly(methacrylic acid-co-ethyl acrylate) 1:1) and nonionic surfactant Brij98. The features of the interaction between Eudragit L100-55 and Brij98 at different pH values and their optimal ratio for nanoparticle formation were studied using isothermal titration calorimetry. The influence of the polymer-to-surfactant ratio on the size and structure of particles was studied at different pH values using dynamic light scattering and small-angle X-ray scattering methods. It was shown that stable nanoparticles are formed at acidic pH at polymer-to-surfactant molar ratios from 1:43 to 1:139. Trypsin was successfully encapsulated into Eudragit-Brij98 nanoparticles as a model bioactive component. The loading efficiency was determined by labeling trypsin with radioactive iodine-125. Eudragit-Brij98 nanoparticles effectively protected trypsin against pepsin digestion. The results showed that trypsin encapsulated into novel pH-sensitive nanocontainers retained more than 50% of its activity after treatment with pepsin compared with nonencapsulated trypsin. The described concept will contribute both to understanding the principles of and designing next-generation nanocontainers
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acrylic Resins
|2 NLM
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|a Drug Carriers
|2 NLM
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|a Eudragit L100-55
|2 NLM
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|a Iodine Radioisotopes
|2 NLM
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|a Plant Oils
|2 NLM
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|a Polyelectrolytes
|2 NLM
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|a Surface-Active Agents
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
|2 NLM
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|a polyethylene glycol oleyl ether
|2 NLM
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|a 9004-98-2
|2 NLM
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|a Trypsin
|2 NLM
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| 650 |
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|a EC 3.4.21.4
|2 NLM
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| 700 |
1 |
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|a Kaberov, Leonid I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kučka, Jan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bogomolova, Anna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stepanek, Petr
|e verfasserin
|4 aut
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|a Filippov, Sergey K
|e verfasserin
|4 aut
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| 700 |
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|a Hruby, Martin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 3 vom: 24. Jan., Seite 764-772
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:33
|g year:2017
|g number:3
|g day:24
|g month:01
|g pages:764-772
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|u http://dx.doi.org/10.1021/acs.langmuir.6b03778
|3 Volltext
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|d 33
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|h 764-772
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