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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw463
|2 doi
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|a pubmed25n0892.xml
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|a (DE-627)NLM267631995
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|a (NLM)28043950
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Denton, Alisandra K
|e verfasserin
|4 aut
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|a Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.02.2018
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|a Date Revised 27.03.2024
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|a published: Print-Electronic
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|a Dryad: 10.5061/dryad.tf6q6
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|a ErratumIn: J Exp Bot. 2018 Jun 27;69(15):3789. doi: 10.1093/jxb/ery196. - PMID 29873761
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a The high efficiency of C4 photosynthesis relies on spatial division of labor, classically with initial carbon fixation in the mesophyll and carbon reduction in the bundle sheath. By employing grinding and serial filtration over liquid nitrogen, we enriched C4 tissues along a developing leaf gradient. This method treats both C4 tissues in an integrity-preserving and consistent manner, while allowing complementary measurements of metabolite abundance and enzyme activity, thus providing a comprehensive data set. Meta-analysis of this and the previous studies highlights the strengths and weaknesses of different C4 tissue separation techniques. While the method reported here achieves the least enrichment, it is the only one that shows neither strong 3' (degradation) bias, nor different severity of 3' bias between samples. The meta-analysis highlighted previously unappreciated observations, such as an accumulation of evidence that aspartate aminotransferase is more mesophyll specific than expected from the current NADP-ME C4 cycle model, and a shift in enrichment of protein synthesis genes from bundle sheath to mesophyll during development. The full comparative dataset is available for download, and a web visualization tool (available at http://www.plant-biochemistry.hhu.de/resources.html) facilitates comparison of the the Z. mays bundle sheath and mesophyll studies, their consistencies and their conflicts
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|a Journal Article
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|a Meta-Analysis
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|a Validation Study
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|a C4
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|a cell separation
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|a maize
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|a meta-analysis
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|a transcriptomics.
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|a Maß, Janina
|e verfasserin
|4 aut
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|a Külahoglu, Canan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lercher, Martin J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bräutigam, Andrea
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Weber, Andreas P M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 2 vom: 15. Jan., Seite 147-160
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:68
|g year:2017
|g number:2
|g day:15
|g month:01
|g pages:147-160
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|u http://dx.doi.org/10.1093/jxb/erw463
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