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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b03309
|2 doi
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|a DE-627
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|a eng
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|a Martin, Michael
|e verfasserin
|4 aut
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|a Planar Bilayer Measurements of Alamethicin and Gramicidin Reconstituted in Biomimetic Block Copolymers
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|c 2017
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 18.04.2018
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|a Date Revised 18.04.2018
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a This work explores methods for forming and characterizing biomimetic planar membranes composed of amphiphilic block copolymers. The membranes are diblocks and triblocks with hydrophilic blocks of poly(2-methyl-2-oxazoline) (PMOXA) and hydrophobic blocks of poly(dimethylsiloxane) (PDMS). Experiments with the lipid diphytanoyl phosphocholine (DPhPC) serve as a basis for comparison with the polymeric membranes. Phase-contrast microscopy is used to study how membranes evolve over time after their formation. Capacitance measurements as a function of the thinned membrane area (prepared from two separate solvent systems) are performed to clarify the importance of the Plateau-Gibbs border in electrical measurements. Finally, functional reconstitution of the two ion channels, alamethicin and gramicidin, is investigated. Imaging in transmitted phase-contrast mode provides visualization of thinned regions that contain monolayers or bilayers (in the case of diblock copolymer). The specific capacitance measurements yield 0.28 μF/cm2 with a corresponding thickness of 8.5 nm for PMOXA6-PDMS35-PMOXA6 (blocks of 6 PMOXA and 35 PDMS repeat units) formed from a solution of ethanol-decane, 0.55 μF/cm2 and 4.4 nm in chloroform-toluene, and 0.46 μF/cm2 and 5.4 nm for the diblock PMOXA6-PDMS17 in ethanol-decane. Alamethicin reconstitution in the block copolymers shows slower channel-forming kinetics with somewhat higher conductance values than found in DPhPC. Gramicidin in the block copolymer shows a slightly voltage-dependent conductance as a function of time, with little stochastic conductance state switching, in contrast to reconstitution in DPhPC where gramicidin switches states at ∼3 Hz
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Dubbs, Timothy
|e verfasserin
|4 aut
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|a Fried, J R
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 33(2017), 5 vom: 07. Feb., Seite 1171-1179
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|x 1520-5827
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|g volume:33
|g year:2017
|g number:5
|g day:07
|g month:02
|g pages:1171-1179
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|u http://dx.doi.org/10.1021/acs.langmuir.6b03309
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