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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2016.12.007
|2 doi
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|a pubmed25n0891.xml
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|a (DE-627)NLM267588127
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|a (NLM)28039017
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|a (PII)S1521-6616(16)30395-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Singhal, Rashi
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.06.2017
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|a Date Revised 06.02.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Inc. All rights reserved.
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|a Monocytes and macrophage combat infections and maintain homeostatic balance by engulfing microbes and apoptotic cells, and releasing inflammatory cytokines. Studies have described that these cells develop anti-inflammatory properties upon recycling the free-hemoglobin (Hb) in hemolytic conditions. While investigating the phenotype of monocytes in two hemolytic disorders-paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD), we observed a high number of pro-inflammatory (CD14+CD16hi) monocytes in these patients. We further investigated in vitro the phenotype of these monocytes and found an estimated 55% of CD14+ cells were transformed into the CD14+CD16hi subset after engulfing Hb-activated platelets. The CD14+CD16hi monocytes, which were positive for both intracellular Hb and CD42b (platelet marker), secreted significant amounts of TNF-α and IL-1β, unlike monocytes treated with only free Hb, which secreted more IL-10. We have shown recently the presence of a high number of Hb-bound hyperactive platelets in patients with both diseases, and further investigated if the monocytes engulfed these activated platelets in vivo. As expected, we found 95% of CD14+CD16hi monocytes with both intracellular Hb and CD42b in both diseases, and they expressed high TNF-α. Furthermore our data showed that these monocytes whether from patients or developed in vitro after treatment with Hb-activated platelets, secreted significant amounts of tissue factor. Besides, these CD14+CD16hi monocytes displayed significantly decreased phagocytosis of E. coli. Our study therefore suggests that this alteration of monocyte phenotype may play a role in the increased propensity to pro-inflammatory/coagulant complications observed in these hemolytic disorders-PNH and SCD
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Coagulation and hemolytic disorders
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|a Hemoglobin
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|a Inflammation
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| 650 |
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|a Monocytes
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|a Platelets
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| 650 |
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|a Hemoglobins
|2 NLM
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| 650 |
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|a Interleukin-1beta
|2 NLM
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| 650 |
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|a Lipopolysaccharide Receptors
|2 NLM
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| 650 |
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|a Platelet Glycoprotein GPIb-IX Complex
|2 NLM
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| 650 |
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|a Receptors, IgG
|2 NLM
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| 650 |
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|a Tumor Necrosis Factor-alpha
|2 NLM
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| 650 |
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|a Interleukin-10
|2 NLM
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| 650 |
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|a 130068-27-8
|2 NLM
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| 700 |
1 |
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|a Chawla, Sheetal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rathore, Deepak K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bhasym, Angika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Annarapu, Gowtham K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sharma, Vandana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Seth, Tulika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guchhait, Prasenjit
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 175(2017) vom: 07. Feb., Seite 133-142
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
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|g volume:175
|g year:2017
|g day:07
|g month:02
|g pages:133-142
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2016.12.007
|3 Volltext
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