P13, the EMBL macromolecular crystallography beamline at the low-emittance PETRA III ring for high- and low-energy phasing with variable beam focusing

The macromolecular crystallography P13 beamline is part of the European Molecular Biology Laboratory Integrated Facility for Structural Biology at PETRA III (DESY, Hamburg, Germany) and has been in user operation since mid-2013. P13 is tunable across the energy range from 4 to 17.5 keV to support cr...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 24(2017), Pt 1 vom: 01. Jan., Seite 323-332
1. Verfasser: Cianci, Michele (VerfasserIn)
Weitere Verfasser: Bourenkov, Gleb, Pompidor, Guillaume, Karpics, Ivars, Kallio, Johanna, Bento, Isabel, Roessle, Manfred, Cipriani, Florent, Fiedler, Stefan, Schneider, Thomas R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2θ-detector arm low-energy data collection phasing softer X-rays sulfur SAD tunability variable beam
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520 |a The macromolecular crystallography P13 beamline is part of the European Molecular Biology Laboratory Integrated Facility for Structural Biology at PETRA III (DESY, Hamburg, Germany) and has been in user operation since mid-2013. P13 is tunable across the energy range from 4 to 17.5 keV to support crystallographic data acquisition exploiting a wide range of elemental absorption edges for experimental phase determination. An adaptive Kirkpatrick-Baez focusing system provides an X-ray beam with a high photon flux and tunable focus size to adapt to diverse experimental situations. Data collections at energies as low as 4 keV (λ = 3.1 Å) are possible due to a beamline design minimizing background and maximizing photon flux particularly at low energy (up to 1011 photons s-1 at 4 keV), a custom calibration of the PILATUS 6M-F detector for use at low energies, and the availability of a helium path. At high energies, the high photon flux (5.4 × 1011 photons s-1 at 17.5 keV) combined with a large area detector mounted on a 2θ arm allows data collection to sub-atomic resolution (0.55 Å). A peak flux of about 8.0 × 1012 photons s-1 is reached at 11 keV. Automated sample mounting is available by means of the robotic sample changer `MARVIN' with a dewar capacity of 160 samples. In close proximity to the beamline, laboratories have been set up for sample preparation and characterization; a laboratory specifically equipped for on-site heavy atom derivatization with a library of more than 150 compounds is available to beamline users 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a 2θ-detector arm 
650 4 |a low-energy data collection 
650 4 |a phasing 
650 4 |a softer X-rays 
650 4 |a sulfur SAD 
650 4 |a tunability 
650 4 |a variable beam 
700 1 |a Bourenkov, Gleb  |e verfasserin  |4 aut 
700 1 |a Pompidor, Guillaume  |e verfasserin  |4 aut 
700 1 |a Karpics, Ivars  |e verfasserin  |4 aut 
700 1 |a Kallio, Johanna  |e verfasserin  |4 aut 
700 1 |a Bento, Isabel  |e verfasserin  |4 aut 
700 1 |a Roessle, Manfred  |e verfasserin  |4 aut 
700 1 |a Cipriani, Florent  |e verfasserin  |4 aut 
700 1 |a Fiedler, Stefan  |e verfasserin  |4 aut 
700 1 |a Schneider, Thomas R  |e verfasserin  |4 aut 
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773 1 8 |g volume:24  |g year:2017  |g number:Pt 1  |g day:01  |g month:01  |g pages:323-332 
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