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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1107/S1600577516018452
|2 doi
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|a pubmed25n0891.xml
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|a (DE-627)NLM267420749
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|a (NLM)28009546
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Crosas, Eva
|e verfasserin
|4 aut
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| 245 |
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|a Uridine as a new scavenger for synchrotron-based structural biology techniques
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.05.2018
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|a Date Revised 02.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of ∼1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5% v/v glycerol, and greater than 2 mM DTT and 1 mM ascorbic acid. In all cases, the protective effect of uridine is proportional to its concentration
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a SAXS methods
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|a free radical scavenger
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| 650 |
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|a macromolecular crystallography methods
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|a radiation damage
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|a reactive oxygen species
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|a Proteins
|2 NLM
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|a Uridine
|2 NLM
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| 650 |
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|a WHI7HQ7H85
|2 NLM
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| 700 |
1 |
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|a Castellvi, Albert
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Crespo, Isidro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fulla, Daniel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gil-Ortiz, Fernando
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fuertes, Gustavo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kamma-Lorger, Christina S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Malfois, Marc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aranda, Miguel A G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Juanhuix, Jordi
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 24(2017), Pt 1 vom: 01. Jan., Seite 53-62
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
1 |
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|g volume:24
|g year:2017
|g number:Pt 1
|g day:01
|g month:01
|g pages:53-62
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|u http://dx.doi.org/10.1107/S1600577516018452
|3 Volltext
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|a AR
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|d 24
|j 2017
|e Pt 1
|b 01
|c 01
|h 53-62
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