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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2016.10.006
|2 doi
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|a pubmed25n0890.xml
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|a (NLM)27964782
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|a (PII)S0168-9452(16)30537-4
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Lin, Chia-Hua
|e verfasserin
|4 aut
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| 245 |
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|a The pathogen-inducible promoter of defense-related LsGRP1 gene from Lilium functioning in phylogenetically distinct species of plants
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 16.03.2017
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
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|a A suitable promoter greatly enhances the efficiency of target gene expression of plant molecular breeding and farming; however, only very few promoters are available for economically important non-graminaceous ornamental monocots. In this study, an 868-bp upstream region of defense-related LsGRP1 of Lilium, named PLsGRP1, was cloned by genome walking and proven to exhibit promoter activity in Nicotiana benthamiana and Lilium 'Stargazer' as assayed by agroinfiltration-based β-glucuronidase (GUS) expression system. Many putative biotic stress-, abiotic stress- and physiological regulation-related cis-acting elements were found in PLsGRP1. Serial deletion analysis of PLsGRP1 performed in Nicotiana tabacum var. Wisconsin 38 accompanied with types of treatments indicated that 868-bp PLsGRP1 was highly induced upon pathogen challenges and cold stress while the 131-bp 3'-end region of PLsGRP1 could be dramatically induced by many kinds of abiotic stresses, biotic stresses and phytohormone treatments. Besides, transient GUS expression in a fern, gymnosperms, monocots and dicots revealed good promotor activity of PLsGRP1 in many phylogenetically distinct plant species. Thus, pathogen-inducible PLsGRP1 and its 131-bp 3'-end region are presumed potential as tools for plant molecular breeding and farming
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|a Journal Article
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|a Agroinfiltration
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|a Inducible promoter
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|a LsGRP1
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|a Non-graminaceous monocot
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|a Plant Proteins
|2 NLM
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|a Glucuronidase
|2 NLM
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|a EC 3.2.1.31
|2 NLM
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| 700 |
1 |
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|a Chen, Chao-Ying
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 254(2017) vom: 14. Jan., Seite 22-31
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:254
|g year:2017
|g day:14
|g month:01
|g pages:22-31
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|u http://dx.doi.org/10.1016/j.plantsci.2016.10.006
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