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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1111/nph.14326
|2 doi
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|a pubmed25n0889.xml
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|a (DE-627)NLM266881459
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|a (NLM)27924627
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Tzafestas, Kyriakos
|e verfasserin
|4 aut
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|a Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.02.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
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|a The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4,6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax ), and five-fold lower Michaelis Constant (Km ) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites
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|a Journal Article
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|a Drosophila melanogaster
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|a 2,4,6-trinitrotoluene
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|a Arabidopsis
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|a conjugation
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|a detoxification
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|a environmental pollutant
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|a glutathione transferase
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|a phytoremediation
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|a Arabidopsis Proteins
|2 NLM
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|a Drosophila Proteins
|2 NLM
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|a Environmental Pollutants
|2 NLM
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|a Explosive Agents
|2 NLM
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|a Trinitrotoluene
|2 NLM
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| 650 |
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|a 118-96-7
|2 NLM
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|a GSTE6 protein, Drosophila
|2 NLM
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| 650 |
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|a EC 2.5.1.18
|2 NLM
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| 650 |
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|a Glutathione Transferase
|2 NLM
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| 650 |
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|a EC 2.5.1.18
|2 NLM
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| 650 |
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|a Glutathione
|2 NLM
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| 650 |
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|a GAN16C9B8O
|2 NLM
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| 700 |
1 |
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|a Razalan, Maria M
|e verfasserin
|4 aut
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1 |
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|a Gyulev, Ivan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mazari, Aslam M A
|e verfasserin
|4 aut
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| 700 |
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|a Mannervik, Bengt
|e verfasserin
|4 aut
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| 700 |
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|a Rylott, Elizabeth L
|e verfasserin
|4 aut
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| 700 |
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|a Bruce, Neil C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1990
|g 214(2017), 1 vom: 06. Apr., Seite 294-303
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:214
|g year:2017
|g number:1
|g day:06
|g month:04
|g pages:294-303
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|u http://dx.doi.org/10.1111/nph.14326
|3 Volltext
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|d 214
|j 2017
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|h 294-303
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