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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2016.11.006
|2 doi
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|a pubmed25n0888.xml
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|a (DE-627)NLM266574742
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|a (NLM)27889517
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|a (PII)S0176-1617(16)30252-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Nguyen, Hong Phuong
|e verfasserin
|4 aut
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|a Rice pectin methylesterase inhibitor28 (OsPMEI28) encodes a functional PMEI and its overexpression results in a dwarf phenotype through increased pectin methylesterification levels
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 25.04.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier GmbH. All rights reserved.
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|a Pectin methylesterases (PMEs, EC 3.1.1.11) belonging to carbohydrate esterase family 8 cleave the ester bond between a galacturonic acid and an methyl group and the resulting change in methylesterification level plays an important role during the growth and development of plants. Optimal pectin methylesterification status in each cell type is determined by the balance between PME activity and post-translational PME inhibition by PME inhibitors (PMEIs). Rice contains 49 PMEIs and none of them are functionally characterized. Genomic sequence analysis led to the identification of rice PMEI28 (OsPMEI28). Recombinant OsPMEI28 exhibited inhibitory activity against commercial PME protein with the highest activities detected at pH 8.5. Overexpression of OsPMEI28 in rice resulted in an increased level of cell wall bound methylester groups and differential changes in the composition of cell wall neutral monosaccharides and lignin content in culm tissues. Consequently, transgenic plants overexpressing OsPMEI28 exhibited dwarf phenotypes and reduced culm diameter. Our data indicate that OsPMEI28 functions as a critical structural modulator by regulating the degree of pectin methylesterification and that an impaired status of pectin methylesterification affects physiochemical properties of the cell wall components and causes abnormal cell extensibility in rice culm tissues
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|a Journal Article
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|a Cell wall modifications
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|a Pectin
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|a Pectin methylesterase inhibitors
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|a Pectin methylesterases
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|a Plant cell walls
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|a Rice
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|a Enzyme Inhibitors
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Recombinant Proteins
|2 NLM
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|a Pectins
|2 NLM
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|a 89NA02M4RX
|2 NLM
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| 650 |
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|a Carboxylic Ester Hydrolases
|2 NLM
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|a EC 3.1.1.-
|2 NLM
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| 650 |
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|a pectinesterase
|2 NLM
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| 650 |
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|a EC 3.1.1.11
|2 NLM
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| 700 |
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|a Jeong, Ho Young
|e verfasserin
|4 aut
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|a Jeon, Seung Ho
|e verfasserin
|4 aut
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| 700 |
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|a Kim, Donghyuk
|e verfasserin
|4 aut
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| 700 |
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|a Lee, Chanhui
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 208(2017) vom: 01. Jan., Seite 17-25
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:208
|g year:2017
|g day:01
|g month:01
|g pages:17-25
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2016.11.006
|3 Volltext
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|d 208
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