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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12425
|2 doi
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|a eng
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1 |
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|a Moller, Cheryl A
|e verfasserin
|4 aut
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|a The hematology of captive Bobtail lizards (Tiliqua rugosa)
|b blood counts, light microscopy, cytochemistry, and ultrastructure
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 09.08.2017
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|a Date Revised 09.08.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2016 American Society for Veterinary Clinical Pathology.
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|a INTRODUCTION: Bobtail lizards (Tiliqua rugosa) are native to Australia. The only previous study on the hematology of this species documented just 6 animals
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|a OBJECTIVES: The aims of this study were to characterize the light microscopy, ultrastructure and cytochemistry of blood cells, and evaluate CBCs of captive Bobtail lizards
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|a METHODS: Over 2 consecutive summers, heparinized venous blood was collected from the ventral coccygeal vein of 46 clinically healthy, captive indoor- or outdoor-housed adult Bobtails. Complete blood cell counts and smear evaluations were performed, and cytochemical stains and transmission electron microscopy were used to further characterize blood cells
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|a RESULTS: The eosinophils of this species were uniformly vacuolated: a unique feature not previously reported in reptiles. Heterophils were the predominant leukocyte, with fewer lymphocytes, azurophilic and nonazurophilic monocytes, occasional eosinophils, and basophils. Thrombocytes were frequently clumped. Slight polychromasia (0-15% of erythrocytes) was typically present. Hemogregarine parasites were seen on some smears. The range of CBC results was often wide. The PCV ranged from 11% to 38%. Total plasma proteins by refractometry were between 3.5 and 7.8 g/dL. Hemoglobin ranged between 2.6 and 12.6 g/dL by the modified hemoglobin-hydroxylamine method. Manual RBC count was 0.35-1.27 × 106 /μL, and WBC count was 2.86-22.66 × 103 /μL. Bobtail lizards housed outdoors had lower PCVs than indoor-housed animals. Bobtails with hemogregarine infections had lower PCVs than noninfected lizards
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|a CONCLUSIONS: Ranges for CBC data were often very wide, influenced by preanalytic and analytic factors. Hemogregarine infection is associated with a decreased PCV, suggesting that some hemogregarine species are pathogenic in this population
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|a Journal Article
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|a Blood cells
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|a hemogregarine
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|a reptile
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| 650 |
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|a vacuolated eosinophils
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1 |
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|a Gaál, Tibor
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mills, Jennifer N
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 45(2016), 4 vom: 01. Dez., Seite 634-647
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|g year:2016
|g number:4
|g day:01
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|g pages:634-647
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