Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus

Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus

© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 214(2017), 1 vom: 18. Apr., Seite 219-232
1. Verfasser: Cormier, Alexandre (VerfasserIn)
Weitere Verfasser: Avia, Komlan, Sterck, Lieven, Derrien, Thomas, Wucher, Valentin, Andres, Gwendoline, Monsoor, Misharl, Godfroy, Olivier, Lipinska, Agnieszka, Perrineau, Marie-Mathilde, Van De Peer, Yves, Hitte, Christophe, Corre, Erwan, Coelho, Susana M, Cock, J Mark
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Ectocarpus Saccharina japonica alternative splicing brown algae genetic markers genome reannotation long noncoding RNAs stramenopile Algal Proteins mehr... DNA, Intergenic RNA, Long Noncoding
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100 1 |a Cormier, Alexandre  |e verfasserin  |4 aut 
245 1 0 |a Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus 
264 1 |c 2017 
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500 |a Date Revised 09.01.2024 
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520 |a © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust. 
520 |a The genome of the filamentous brown alga Ectocarpus was the first to be completely sequenced from within the brown algal group and has served as a key reference genome both for this lineage and for the stramenopiles. We present a complete structural and functional reannotation of the Ectocarpus genome. The large-scale assembly of the Ectocarpus genome was significantly improved and genome-wide gene re-annotation using extensive RNA-seq data improved the structure of 11 108 existing protein-coding genes and added 2030 new loci. A genome-wide analysis of splicing isoforms identified an average of 1.6 transcripts per locus. A large number of previously undescribed noncoding genes were identified and annotated, including 717 loci that produce long noncoding RNAs. Conservation of lncRNAs between Ectocarpus and another brown alga, the kelp Saccharina japonica, suggests that at least a proportion of these loci serve a function. Finally, a large collection of single nucleotide polymorphism-based markers was developed for genetic analyses. These resources are available through an updated and improved genome database. This study significantly improves the utility of the Ectocarpus genome as a high-quality reference for the study of many important aspects of brown algal biology and as a reference for genomic analyses across the stramenopiles 
650 4 |a Journal Article 
650 4 |a Ectocarpus 
650 4 |a Saccharina japonica 
650 4 |a alternative splicing 
650 4 |a brown algae 
650 4 |a genetic markers 
650 4 |a genome reannotation 
650 4 |a long noncoding RNAs 
650 4 |a stramenopile 
650 7 |a Algal Proteins  |2 NLM 
650 7 |a DNA, Intergenic  |2 NLM 
650 7 |a RNA, Long Noncoding  |2 NLM 
700 1 |a Avia, Komlan  |e verfasserin  |4 aut 
700 1 |a Sterck, Lieven  |e verfasserin  |4 aut 
700 1 |a Derrien, Thomas  |e verfasserin  |4 aut 
700 1 |a Wucher, Valentin  |e verfasserin  |4 aut 
700 1 |a Andres, Gwendoline  |e verfasserin  |4 aut 
700 1 |a Monsoor, Misharl  |e verfasserin  |4 aut 
700 1 |a Godfroy, Olivier  |e verfasserin  |4 aut 
700 1 |a Lipinska, Agnieszka  |e verfasserin  |4 aut 
700 1 |a Perrineau, Marie-Mathilde  |e verfasserin  |4 aut 
700 1 |a Van De Peer, Yves  |e verfasserin  |4 aut 
700 1 |a Hitte, Christophe  |e verfasserin  |4 aut 
700 1 |a Corre, Erwan  |e verfasserin  |4 aut 
700 1 |a Coelho, Susana M  |e verfasserin  |4 aut 
700 1 |a Cock, J Mark  |e verfasserin  |4 aut 
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773 1 8 |g volume:214  |g year:2017  |g number:1  |g day:18  |g month:04  |g pages:219-232 
856 4 0 |u http://dx.doi.org/10.1111/nph.14321  |3 Volltext 
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