A simple enrichment procedure improves detection of membrane proteins by immunoblotting
We developed a novel approach to improve detection of membrane-associated proteins in yeast cell lysates by immunoblotting. Our method consists of a simple enrichment procedure using sedimentation to remove soluble proteins and the use of an alternative electrophoresis sample buffer, which allows fo...
| Publié dans: | BioTechniques. - 1991. - 61(2016), 5 vom: 01., Seite 260-261 |
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| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2016
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article gel electrophoresis immunoblotting membrane protein protein extraction yeast Fungal Proteins Membrane Proteins |