Accurate quantitative wide-field fluorescence microscopy of 3-D spheroids

Hundreds of commercially available fluorescent dyes are used to quantify a wide range of biological functions of cells in culture, and their use has been a mainstay of basic research, toxicity testing, and drug discovery. However, nearly all of these dyes have been optimized for use on cells culture...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 61(2016), 5 vom: 01., Seite 237-247
Auteur principal: Leary, Elizabeth (Auteur)
Autres auteurs: Rhee, Claire, Wilks, Benjamin, Morgan, Jeffrey R
Format: Article en ligne
Langue:English
Publié: 2016
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't calcein-AM epi-fluorescence live cell imaging quantitative image analysis spheroids Fluorescent Dyes Hydrogels