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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw414
|2 doi
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|a eng
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|a Arrivault, Stéphanie
|e verfasserin
|4 aut
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|a Metabolite pools and carbon flow during C4 photosynthesis in maize
|b 13CO2 labeling kinetics and cell type fractionation
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.02.2018
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|a Date Revised 27.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Worldwide efforts to engineer C4 photosynthesis into C3 crops require a deep understanding of how this complex pathway operates. CO2 is incorporated into four-carbon metabolites in the mesophyll, which move to the bundle sheath where they are decarboxylated to concentrate CO2 around RuBisCO. We performed dynamic 13CO2 labeling in maize to analyze C flow in C4 photosynthesis. The overall labeling kinetics reflected the topology of C4 photosynthesis. Analyses of cell-specific labeling patterns after fractionation to enrich bundle sheath and mesophyll cells revealed concentration gradients to drive intercellular diffusion of malate, but not pyruvate, in the major CO2-concentrating shuttle. They also revealed intercellular concentration gradients of aspartate, alanine, and phosphenolpyruvate to drive a second phosphoenolpyruvate carboxykinase (PEPCK)-type shuttle, which carries 10-14% of the carbon into the bundle sheath. Gradients also exist to drive intercellular exchange of 3-phosphoglycerate and triose-phosphate. There is rapid carbon exchange between the Calvin-Benson cycle and the CO2-concentrating shuttle, equivalent to ~10% of carbon gain. In contrast, very little C leaks from the large pools of metabolites in the C concentration shuttle into respiratory metabolism. We postulate that the presence of multiple shuttles, alongside carbon transfer between them and the Calvin-Benson cycle, confers great flexibility in C4 photosynthesis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 13C labeling
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|a C4 photosynthesis
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|a CO2-concentrating shuttle
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|a carbon flow
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|a maize.
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|a Carbon Isotopes
|2 NLM
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|a Carbon Dioxide
|2 NLM
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|a 142M471B3J
|2 NLM
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|a Oxygen
|2 NLM
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| 650 |
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|a S88TT14065
|2 NLM
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| 700 |
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|a Obata, Toshihiro
|e verfasserin
|4 aut
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| 700 |
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|a Szecówka, Marek
|e verfasserin
|4 aut
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| 700 |
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|a Mengin, Virginie
|e verfasserin
|4 aut
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|a Guenther, Manuela
|e verfasserin
|4 aut
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|a Hoehne, Melanie
|e verfasserin
|4 aut
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| 700 |
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|a Fernie, Alisdair R
|e verfasserin
|4 aut
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| 700 |
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|a Stitt, Mark
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 2 vom: 29. Jan., Seite 283-298
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:68
|g year:2017
|g number:2
|g day:29
|g month:01
|g pages:283-298
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|u http://dx.doi.org/10.1093/jxb/erw414
|3 Volltext
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