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|a DE-627
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|a eng
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| 100 |
1 |
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|a Alvarez, Ashley Ann
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Wortmannin-induced vacuole fusion enhances amyloplast dynamics in Arabidopsis zigzag1 hypocotyls
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|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 21.11.2017
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|a Date Revised 02.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Gravitropism in Arabidopsis shoots depends on the sedimentation of amyloplasts in the endodermis, and a complex interplay between the vacuole and F-actin. Gravity response is inhibited in zigzag-1 (zig-1), a mutant allele of VTI11, which encodes a SNARE protein involved in vacuole fusion. zig-1 seedlings have fragmented vacuoles that fuse after treatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase, and underscore a role of phosphoinositides in vacuole fusion. Using live-cell imaging with a vertical stage microscope, we determined that young endodermal cells below the apical hook that are smaller than 70 μm in length are the graviperceptive cells in dark-grown hypocotyls. This result was confirmed by local wortmannin application to the top of zig-1 hypocotyls, which enhanced shoot gravitropism in zig-1 mutants. Live-cell imaging of zig-1 hypocotyl endodermal cells indicated that amyloplasts are trapped between juxtaposed vacuoles and their movement is severely restricted. Wortmannin-induced fusion of vacuoles in zig-1 seedlings increased the formation of transvacuolar strands, enhanced amyloplast sedimentation and partially suppressed the agravitropic phenotype of zig-1 seedlings. Hypergravity conditions at 10 g were not sufficient to displace amyloplasts in zig-1, suggesting the existence of a physical tether between the vacuole and amyloplasts. Our results overall suggest that vacuole membrane remodeling may be involved in regulating the association of vacuoles and amyloplasts during graviperception
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|a Journal Article
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|a Amyloplasts
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|a SNARE
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|a cytoskeleton
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|a gravitropism
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|a vacuole
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|a wortmannin.
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|a Androstadienes
|2 NLM
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Qb-SNARE Proteins
|2 NLM
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| 650 |
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|a vesicle transport v-SNARE 11 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Wortmannin
|2 NLM
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| 650 |
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|a XVA4O219QW
|2 NLM
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| 700 |
1 |
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|a Han, Sang Won
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Toyota, Masatsugu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brillada, Carla
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Jiameng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gilroy, Simon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rojas-Pierce, Marcela
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 22 vom: 01. Dez., Seite 6459-6472
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|x 1460-2431
|7 nnns
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| 773 |
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|g volume:67
|g year:2016
|g number:22
|g day:01
|g month:12
|g pages:6459-6472
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