|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM265904765 |
| 003 |
DE-627 |
| 005 |
20231227125458.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n1223.xml
|
| 035 |
|
|
|a (DE-627)NLM265904765
|
| 035 |
|
|
|a (NLM)27811086
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Barabasz, Anna
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.11.2017
|
| 500 |
|
|
|a Date Revised 13.12.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a This study links changes in the tobacco endogenous metal-homeostasis network caused by transgene expression with engineering of novel features. It also provides insight into the concentration-dependent mutual interactions between Zn and Cd, leading to differences in the metal partitioning between wild-type and transgenic plants. In tobacco, expression of the export protein AtHMA4 modified Zn/Cd root/shoot distribution, but the pattern depended on their concentrations in the medium. To address this phenomenon, the expression of genes identified by suppression subtractive hybridization and the Zn/Cd accumulation pattern were examined upon exposure to six variants of low/high Zn and Cd concentrations. Five tobacco metal-homeostasis genes were identified: NtZIP2, NtZIP4, NtIRT1-like, NtNAS, and NtVTL. In the wild type, their expression depended on combinations of low/high Zn and Cd concentrations; co-ordinated responses of NtZIP1, NtZIP2, and NtVTL were shown in medium containing 4 µM Cd, and at 0.5 µM versus 10 µM Zn. In transgenics, qualitative changes detected for NtZIP1, NtZIP4, NtIRT1-like, and NtVTL are considered crucial for modification of Zn/Cd supply-dependent Zn/Cd root/shoot distribution. Notwithstanding, NtVTL was the most responsive gene in wild-type and transgenic plants under all concentrations of Zn and Cd tested; thus it is a candidate gene for the regulation of metal cross-homeostasis processes involved in engineering new metal-related traits
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a AtHMA4
|
| 650 |
|
4 |
|a SSH
|
| 650 |
|
4 |
|a cadmium
|
| 650 |
|
4 |
|a tobacco
|
| 650 |
|
4 |
|a transformation
|
| 650 |
|
4 |
|a zinc.
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Cation Transport Proteins
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Cadmium
|2 NLM
|
| 650 |
|
7 |
|a 00BH33GNGH
|2 NLM
|
| 650 |
|
7 |
|a Adenosine Triphosphatases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.6.1.-
|2 NLM
|
| 650 |
|
7 |
|a HMA4 protein, Arabidopsis
|2 NLM
|
| 650 |
|
7 |
|a EC 3.6.1.3
|2 NLM
|
| 650 |
|
7 |
|a Zinc
|2 NLM
|
| 650 |
|
7 |
|a J41CSQ7QDS
|2 NLM
|
| 700 |
1 |
|
|a Klimecka, Maria
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kendziorek, Maria
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Weremczuk, Aleksandra
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ruszczyńska, Anna
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bulska, Ewa
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Antosiewicz, Danuta Maria
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 21 vom: 14. Nov., Seite 6201-6214
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:67
|g year:2016
|g number:21
|g day:14
|g month:11
|g pages:6201-6214
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 67
|j 2016
|e 21
|b 14
|c 11
|h 6201-6214
|