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231224s2016 xx |||||o 00| ||eng c |
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|a pubmed24n1253.xml
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|a (DE-627)NLM265904676
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|a (NLM)27811078
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Torode, Thomas A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Dynamics of cell wall assembly during early embryogenesis in the brown alga Fucus
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| 264 |
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1 |
|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 13.11.2017
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| 500 |
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|a Date Revised 09.01.2024
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| 500 |
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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| 520 |
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|a Zygotes from Fucus species have been used extensively to study cell polarization and rhizoid outgrowth, and in this model system cell wall deposition aligns with the establishment of polarity. Monoclonal antibodies are essential tools for the in situ analysis of cell wall glycans, and here we report the characteristics of six monoclonal antibodies to alginates (BAM6-BAM11). The use of these, in conjunction with monoclonal antibodies to brown algal sulfated fucans, has enabled the study of the developmental dynamics of the Fucus zygote cell walls. Young zygotes are spherical and all alginate epitopes are deposited uniformly following cellulose deposition. At germination, sulfated fucans are secreted in the growing rhizoid wall. The redistribution of cell wall epitopes was investigated during treatments that cause reorientation of the growth axis (change in light direction) or disrupt rhizoid development (arabinogalactan-protein-reactive Yariv reagent). Alginate modeling was drastically impaired in the latter, and both treatments cause a redistribution of highly sulfated fucan epitopes. The dynamics of cell wall glycans in this system have been visualized in situ for the first time, leading to an enhanced understanding of the early developmental mechanisms of Fucus species. These sets of monoclonal antibodies significantly extend the available molecular tools for brown algal cell wall studies
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Alginate
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| 650 |
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4 |
|a Fucus serratus
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| 650 |
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4 |
|a arabinogalactan-protein
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| 650 |
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4 |
|a brown algae
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| 650 |
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4 |
|a cell wall
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| 650 |
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4 |
|a monoclonal antibody
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| 650 |
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4 |
|a sulfated fucan
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| 650 |
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4 |
|a zygote.
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| 650 |
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7 |
|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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7 |
|a Epitopes
|2 NLM
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| 700 |
1 |
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|a Siméon, Amandine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Marcus, Susan E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jam, Murielle
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Le Moigne, Marie-Anne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Duffieux, Delphine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Knox, J Paul
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hervé, Cécile
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 21 vom: 23. Nov., Seite 6089-6100
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:67
|g year:2016
|g number:21
|g day:23
|g month:11
|g pages:6089-6100
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 67
|j 2016
|e 21
|b 23
|c 11
|h 6089-6100
|