Multi-lineages of Shiikuwasha (Citrus depressa Hayata) evaluated by using whole chloroplast genome sequences and its bio-diversity in Okinawa, Japan

Shiikuwasha (Citrus depressa Hayata) is distributed from the South-west of the Japanese archipelago to Taiwan. In this study, re-sequencing against the orange (C. sinensis (L.) Osbeck) chloroplast genome was applied to one superior landrace of Shiikuwasha cultivated in Oku ward, Okinawa, Japan. The...

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Veröffentlicht in:Breeding science. - 1998. - 66(2016), 4 vom: 28. Sept., Seite 490-498
1. Verfasser: Ishikawa, Ryuji (VerfasserIn)
Weitere Verfasser: Badenoch, Nathan, Miyagi, Kunimasa, Medoruma, Kaname, Osada, Toshiki, Onishi, Masayuki
Format: Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Citrus depressa Hayata Shiikuwasha maternal lineage re-sequencing selection
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520 |a Shiikuwasha (Citrus depressa Hayata) is distributed from the South-west of the Japanese archipelago to Taiwan. In this study, re-sequencing against the orange (C. sinensis (L.) Osbeck) chloroplast genome was applied to one superior landrace of Shiikuwasha cultivated in Oku ward, Okinawa, Japan. The chloroplast genome of the landrace was estimated to comprise 160,118 bp, including 48 indels and 71 nucleotide substitutions against the reference genome. The presumptive chloroplast indels were confirmed by subsequent experiments, and these identified multiple maternal lineages among other landraces. Some of the orange SSR markers were available for genotyping of other superior landraces and were able to distinguish among them. These molecular markers were then applied for evaluation of genetic diversity among wild and cultivated Shiikuwasha accessions. Except for Oku ward, the cultivated populations were found to have lost their genetic diversity in comparison with wild populations. Groves in Oku ward maintained, or showed even higher genetic diversity than wild accessions in the surrounding areas by the force of villagers 
650 4 |a Journal Article 
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650 4 |a maternal lineage 
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700 1 |a Miyagi, Kunimasa  |e verfasserin  |4 aut 
700 1 |a Medoruma, Kaname  |e verfasserin  |4 aut 
700 1 |a Osada, Toshiki  |e verfasserin  |4 aut 
700 1 |a Onishi, Masayuki  |e verfasserin  |4 aut 
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