A new method to estimate photosynthetic parameters through net assimilation rate-intercellular space CO2 concentration (A-Ci ) curve and chlorophyll fluorescence measurements
© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
| Publié dans: | The New phytologist. - 1979. - 213(2017), 3 vom: 01. Feb., Seite 1543-1554 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2017
|
| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article A-Ci curves chlorophyll fluorescence fitting method mesophyll conductance photosynthetic parameters Phosphates Chlorophyll 1406-65-1 Carbon Dioxide plus... |
| Résumé: | © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust. Gas exchange (GE) and chlorophyll fluorescence (CF) measurements are widely used to noninvasively study photosynthetic parameters, for example the rates of maximum Rubisco carboxylation (Vcmax ), electron transport rate (J), daytime respiration (Rd ) and mesophyll conductance (gm ). Existing methods for fitting GE data (net assimilation rate-intercellular space CO2 concentration (A-Ci ) curve) are based on two assumptions: gm is unvaried with CO2 concentration in the intercellular space (Ci ); and light absorption (α) and the proportion of quanta absorbed by photosystem II (β) are constant in the data set. These may result in significant bias in estimating photosynthetic parameters. To avoid the above-mentioned hypotheses, we present a new method for fitting A-Ci curves and CF data simultaneously. This method was applied to a data set obtained from cucumber (Cucumis sativus) leaves of various leaf ages and grown under eight different light conditions. The new method had significantly lower root mean square error and a lower rate of failures compared with previously published methods (6.72% versus 24.1%, respectively) and the effect of light conditions on Vcmax and J was better observed. Furthermore, the new method allows the estimation of a new parameter, the fraction of incoming irradiance harvested by photosystem II, and the dependence of gm on Ci |
|---|---|
| Description: | Date Completed 12.02.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.14260 |