Optimization of Diamond Nucleic Acid Dye for quantitative PCR

Here, we evaluate Diamond Nucleic Acid Dye (DD) for use in quantitative PCR (qPCR) applications. Although DD is a commercially available stain for detection of DNA separated by gel electrophoresis, its use as a detection dye in qPCR has yet to be described. To determine if DD can be used in qPCR, we...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 61(2016), 4 vom: 01. Okt., Seite 183-189
1. Verfasser: Haines, Alicia M (VerfasserIn)
Weitere Verfasser: Tobe, Shanan S, Linacre, Adrian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Diamond nucleic acid dye Real-time PCR SYBR Green quantitative PCR (qPCR) Fluorescent Dyes DNA 9007-49-2