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231224s2016 xx |||||o 00| ||eng c |
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|a pubmed24n0883.xml
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|a (DE-627)NLM264989546
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|a (NLM)27702997
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Fei, Qili
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Dynamic changes of small RNAs in rice spikelet development reveal specialized reproductive phasiRNA pathways
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|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 13.11.2017
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| 500 |
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|a Date Revised 31.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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| 520 |
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|a Dissection of the genetic pathways and mechanisms by which anther development occurs in grasses is crucial for both a basic understanding of plant development and for examining traits of agronomic importance such as male sterility. In rice, MULTIPLE SPOROCYTES1 (MSP1), a leucine-rich-repeat receptor kinase, plays an important role in anther development by limiting the number of sporocytes. OsTDL1a (a TPD1-like gene in rice) encodes a small protein that acts as a cofactor of MSP1 in the same regulatory pathway. In this study, we analyzed small RNA and mRNA changes in different stages of spikelets from wild-type rice, and from msp1 and ostdl1a mutants. Analysis of the small RNA data identified miRNAs demonstrating differential abundances. miR2275 was depleted in the two rice mutants; this miRNA is specifically enriched in anthers and functions to trigger the production of 24-nt phased secondary siRNAs (phasiRNAs) from PHAS loci. We observed that the 24-nt phasiRNAs as well as their precursor PHAS mRNAs were also depleted in the two mutants. An analysis of co-expression identified three Argonaute-encoding genes (OsAGO1d, OsAGO2b, and OsAGO18) that accumulate transcripts coordinately with phasiRNAs, suggesting a functional relationship. By mRNA in situ analysis, we demonstrated a strong correlation between the spatiotemporal pattern of these OsAGO transcripts and phasiRNA accumulations
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a Anther
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| 650 |
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4 |
|a Argonaute
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| 650 |
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4 |
|a microRNA
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| 650 |
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4 |
|a phasiRNA
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| 650 |
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|a rice
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| 650 |
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4 |
|a spikelet.
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| 650 |
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7 |
|a Argonaute Proteins
|2 NLM
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| 650 |
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7 |
|a MicroRNAs
|2 NLM
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| 650 |
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7 |
|a RNA, Plant
|2 NLM
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| 650 |
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7 |
|a RNA, Small Interfering
|2 NLM
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| 700 |
1 |
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|a Yang, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liang, Wanqi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Dabing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meyers, Blake C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 21 vom: 01. Nov., Seite 6037-6049
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:67
|g year:2016
|g number:21
|g day:01
|g month:11
|g pages:6037-6049
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|d 67
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|e 21
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|c 11
|h 6037-6049
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