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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b02591
|2 doi
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|a pubmed24n0882.xml
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|a eng
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| 100 |
1 |
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|a Liu, Eric Y
|e verfasserin
|4 aut
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| 245 |
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|a Improved Protein Conjugation with Uniform, Macroporous Poly(acrylamide-co-acrylic acid) Hydrogel Microspheres via EDC/NHS Chemistry
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a We demonstrate a robust and tunable micromolding method to fabricate chemically functional poly(acrylamide-co-acrylic acid) (p(AAm-co-AA)) hydrogel microspheres with uniform dimensions and controlled porous network structures for rapid biomacromolecular conjugation. Specifically, p(AAm-co-AA) microspheres with abundant carboxylate functional groups are fabricated via surface-tension-induced droplet formation in patterned poly(dimethylsiloxane) molds and photoinduced radical polymerization. To demonstrate the chemical functionality, we enlisted rapid EDC/NHS (1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS)) chemistry for fluorescent labeling of the microspheres with small-molecule dye fluorescein glycine amide. Epifluorescence imaging results illustrate the uniform incorporation of carboxylate groups within the microspheres and rapid conjugation kinetics. Furthermore, protein conjugation results using red fluorescent protein R-phycoerythrin demonstrate the highly porous nature of the microspheres as well as the utility of the microspheres and the EDC/NHS scheme for facile biomacromolecular conjugation. Combined, these results illustrate the significant potential for our fabrication-conjugation strategy in the development of biofunctionalized polymeric hydrogel microparticles toward rapid biosensing, bioprocess monitoring, and biodiagnostics
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|a Journal Article
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1 |
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|a Jung, Sukwon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yi, Hyunmin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 42 vom: 25. Okt., Seite 11043-11054
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:32
|g year:2016
|g number:42
|g day:25
|g month:10
|g pages:11043-11054
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|u http://dx.doi.org/10.1021/acs.langmuir.6b02591
|3 Volltext
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