Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency

Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency

Copyright © 2016 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 175(2017) vom: 28. Feb., Seite 124-132
1. Verfasser: Yazdani, Reza (VerfasserIn)
Weitere Verfasser: Ganjalikhani-Hakemi, Mazdak, Esmaeili, Mohammad, Abolhassani, Hassan, Vaeli, Shahram, Rezaei, Abbas, Sharifi, Zohre, Azizi, Gholamreza, Rezaei, Nima, Aghamohammadi, Asghar
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Antibody response B-cell Common variable immunodeficiency PI3K/Akt/FoxO pathway Phosphorylated Akt Phosphatidylinositol 3-Kinases EC 2.7.1.- Proto-Oncogene Proteins c-akt EC 2.7.11.1
Beschreibung
Zusammenfassung:Copyright © 2016 Elsevier Inc. All rights reserved.
Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infections. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA and protein expression of PI3K, Akt and FoxO using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (pAkt) expression in B-cells has been measured by flowcytometry. We identified a significant reduction in the percentage of marginal zone like B-cells, memory B-cells (total, switched and unswitched) and plasmablasts in patients, as these decreased B-cell subsets had a significant negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased pAkt expression in B-cells of patients than HDs. We described for the first time impaired pAkt expression in B-cells of CVID patients that had a significant correlation with antibody response to the vaccine and worse clinical complications
Beschreibung:Date Completed 05.06.2017
Date Revised 06.02.2018
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2016.09.009