Use of real-time PCR with propidium monoazide for enumeration of viable Escherichia coli in anaerobic digestion
A combination of propidium monoazide (PMA) with real-time quantitative polymerase chain reaction (PMA-qPCR) was optimized to enumerate only viable Escherichia coli in anaerobic digestion processes. Repeating the PMA treatment twice and a final concentration of 100 μM resulted in an effective exclusi...
Veröffentlicht in: | Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 74(2016), 5 vom: 20., Seite 1243-54 |
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Format: | Online-Aufsatz |
Sprache: | English |
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2016
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Zugriff auf das übergeordnete Werk: | Water science and technology : a journal of the International Association on Water Pollution Research |
Schlagworte: | Journal Article Azides propidium monoazide Propidium 36015-30-2 |
Online verfügbar |
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