|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM264433084 |
| 003 |
DE-627 |
| 005 |
20231224210057.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0881.xml
|
| 035 |
|
|
|a (DE-627)NLM264433084
|
| 035 |
|
|
|a (NLM)27639094
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Hua, Yingpeng
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Physiological, genomic and transcriptional diversity in responses to boron deficiency in rapeseed genotypes
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 20.11.2017
|
| 500 |
|
|
|a Date Revised 13.11.2018
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Allotetraploid rapeseed (Brassica napus L. AnAnCnCn, 2n=4x=38) is highly susceptible to boron (B) deficiency, a widespread limiting factor that causes severe losses in seed yield. The genetic variation in the sensitivity to B deficiency found in rapeseed genotypes emphasizes the complex response architecture. In this research, a B-inefficient genotype, 'Westar 10' ('W10'), responded to B deficiencies during vegetative and reproductive development with an over-accumulation of reactive oxygen species, severe lipid peroxidation, evident plasmolysis, abnormal floral organogenesis, and widespread sterility compared to a B-efficient genotype, 'Qingyou 10' ('QY10'). Whole-genome re-sequencing (WGS) of 'QY10' and 'W10' revealed a total of 1 605 747 single nucleotide polymorphisms and 218 755 insertions/deletions unevenly distributed across the allotetraploid rapeseed genome (~1130Mb). Digital gene expression (DGE) profiling identified more genes related to B transporters, antioxidant enzymes, and the maintenance of cell walls and membranes with higher transcript levels in the roots of 'QY10' than in 'W10' under B deficiency. Furthermore, based on WGS and bulked segregant analysis of the doubled haploid (DH) line pools derived from 'QY10' and 'W10', two significant quantitative trait loci (QTLs) for B efficiency were characterized on chromosome C2, and DGE-assisted QTL-seq analyses then identified a nodulin 26-like intrinsic protein gene and an ATP-binding cassette (ABC) transporter gene as the corresponding candidates regulating B efficiency. This research facilitates a more comprehensive understanding of the differential physiological and transcriptional responses to B deficiency and abundant genetic diversity in rapeseed genotypes, and the DGE-assisted QTL-seq analyses provide novel insights regarding the rapid dissection of quantitative trait genes in plant species with complex genomes
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a B-deficiency phenotype
|
| 650 |
|
4 |
|a Brassica napus
|
| 650 |
|
4 |
|a boron (B) efficiency
|
| 650 |
|
4 |
|a differentially expressed genes
|
| 650 |
|
4 |
|a genomic variations
|
| 650 |
|
4 |
|a next-generation sequencing.
|
| 650 |
|
7 |
|a Reactive Oxygen Species
|2 NLM
|
| 650 |
|
7 |
|a Boron
|2 NLM
|
| 650 |
|
7 |
|a N9E3X5056Q
|2 NLM
|
| 700 |
1 |
|
|a Zhou, Ting
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ding, Guangda
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Qingyong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Shi, Lei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xu, Fangsen
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 19 vom: 01. Okt., Seite 5769-5784
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:67
|g year:2016
|g number:19
|g day:01
|g month:10
|g pages:5769-5784
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 67
|j 2016
|e 19
|b 01
|c 10
|h 5769-5784
|