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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2016.09.006
|2 doi
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|a pubmed24n0881.xml
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|a (DE-627)NLM264394070
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|a (NLM)27634430
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|a (PII)S1521-6616(16)30376-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wahid, Rezwanul
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a
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|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 31.05.2017
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|a Date Revised 13.11.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Inc. All rights reserved.
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|a The live oral typhoid vaccine Ty21a elicits predominantly CD8+, as well as CD4+ T cells mediated immune responses. Clinical field studies showed that Ty21a is moderately effective against S. Typhi and S. Paratyphi B, but not S. Paratyphi A infections. In this study we describe the in depth characterization of S. Typhi, S. Paratyphi A and S. Paratyphi B cross-reactive CD4+ T cell responses elicited following immunization with Ty21a. PBMC samples were collected from 16 healthy volunteers before and 42/84days after Ty21a immunization and stimulated ex-vivo with Salmonella-infected targets. Multiparametric flow cytometry was used to detect the vaccine elicited Salmonella-specific responses in T effector/memory (TEM) and CD45RA+ T effector/memory (TEMRA) CD4+ cell subsets, by measuring CD4+ multifunctional (MF) cells that concomitantly produced IFN-γ, TNF-α, IL-2, MIP-1β, IL-17A and/or expressed CD107a. Post-vaccination increases in S. Typhi-specific MF cells were observed in CD4+ TEM and TEMRA subsets which predominantly produced IFN-γ and/or TNF-α, while IL-2 was produced by a smaller cell subset. A small proportion of those MF cells also produced MIP-1β, IL-17A and expressed CD107a (a marker associated with cytotoxicity). Approximately one third of these specific MF cells have the potential to migrate to the gut mucosa, as evidenced by co-expression of the gut-homing molecule integrin α4β7. In contrast to our previous observations with CD8+ T cells, MF CD4+ T cell responses to the different Salmonella serovars evaluated were similar in magnitude and characteristics. We conclude that although induction of cross-reactive CD4+ MF effector T cells suggest a possible role in Salmonella-immunity, these responses are unlikely to provide an immunological basis for the observed efficacy of Ty21a against S. Typhi and S. Paratyphi B, but not to S. Paratyphi A
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|a Journal Article
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|a CD4 T cells
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|a Cross-reactive
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|a Human
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|a Multifunctional
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4 |
|a Salmonella
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| 650 |
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4 |
|a Ty21a
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| 650 |
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7 |
|a Cytokines
|2 NLM
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| 650 |
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7 |
|a Polysaccharides, Bacterial
|2 NLM
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| 650 |
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7 |
|a Ty21a typhoid vaccine
|2 NLM
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| 650 |
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7 |
|a Typhoid-Paratyphoid Vaccines
|2 NLM
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| 650 |
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7 |
|a Vaccines, Live, Unattenuated
|2 NLM
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| 700 |
1 |
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|a Fresnay, Stephanie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Levine, Myron M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sztein, Marcelo B
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 173(2016) vom: 15. Dez., Seite 87-95
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:173
|g year:2016
|g day:15
|g month:12
|g pages:87-95
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2016.09.006
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 173
|j 2016
|b 15
|c 12
|h 87-95
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