Batch and fed-batch simultaneous saccharification and fermentation of primary sludge from pulp and paper mills

Primary sludge from a Portuguese pulp and paper mill, containing 60% of carbohydrates, and unbleached pulp (as reference material), with 93% of carbohydrates, were used to produce ethanol by simultaneous saccharification and fermentation (SSF). SSF was performed in batch or fed-batch conditions with...

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Veröffentlicht in:Environmental technology. - 1998. - 38(2017), 12 vom: 01. Juni, Seite 1498-1506
1. Verfasser: Mendes, Cátia Vanessa Teixeira (VerfasserIn)
Weitere Verfasser: Rocha, Jorge Manuel Dos Santos, de Menezes, Fabrícia Farias, Carvalho, Maria da Graça Videira Sousa
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Batch ethanol fed-batch primary sludge simultaneous saccharification fermentation Industrial Waste Ethanol 3K9958V90M Xylitol VCQ006KQ1E
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520 |a Primary sludge from a Portuguese pulp and paper mill, containing 60% of carbohydrates, and unbleached pulp (as reference material), with 93% of carbohydrates, were used to produce ethanol by simultaneous saccharification and fermentation (SSF). SSF was performed in batch or fed-batch conditions without the need of a pretreatment. Cellic® CTec2 was the cellulolytic enzymatic complex used and Saccharomyces cerevisiae (baker's yeast or ATCC 26602 strain) or the thermotolerant yeast Kluyveromyces marxianus NCYC 1426 were employed. Primary sludge was successfully converted to ethanol and the best results in SSF efficiency were obtained with S. cerevisiae. An ethanol concentration of 22.7 g L-1 was produced using a content of 50 g L-1 of carbohydrates from primary sludge, in batch conditions, with a global conversion yield of 81% and a production rate of 0.94 g L-1 h-1. Fed-batch operation enabled higher solids content (total carbohydrate concentration of 200 g L-1, equivalent to a consistency of 33%) and a reduction of three-quarters of cellulolytic enzyme load, leading to an ethanol concentration of 40.7 g L-1, although with lower yield and productivity. Xylitol with a concentration up to 7 g L-1 was also identified as by-product in the primary sludge bioconversion process 
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650 4 |a Batch 
650 4 |a ethanol 
650 4 |a fed-batch 
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650 4 |a simultaneous saccharification fermentation 
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650 7 |a Ethanol  |2 NLM 
650 7 |a 3K9958V90M  |2 NLM 
650 7 |a Xylitol  |2 NLM 
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700 1 |a Rocha, Jorge Manuel Dos Santos  |e verfasserin  |4 aut 
700 1 |a de Menezes, Fabrícia Farias  |e verfasserin  |4 aut 
700 1 |a Carvalho, Maria da Graça Videira Sousa  |e verfasserin  |4 aut 
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