|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM26417612X |
| 003 |
DE-627 |
| 005 |
20231224205518.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.clim.2016.09.001
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0880.xml
|
| 035 |
|
|
|a (DE-627)NLM26417612X
|
| 035 |
|
|
|a (NLM)27609500
|
| 035 |
|
|
|a (PII)S1521-6616(16)30359-X
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Wang, Jing J
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a IgV peptide mapping of native Ro60 autoantibody proteomes in primary Sjögren's syndrome reveals molecular markers of Ro/La diversification
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 31.05.2017
|
| 500 |
|
|
|a Date Revised 14.12.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2016 Elsevier Inc. All rights reserved.
|
| 520 |
|
|
|a We have used high-resolution mass spectrometry to sequence precipitating anti-Ro60 proteomes from sera of patients with primary Sjögren's syndrome and compare immunoglobulin variable-region (IgV) peptide signatures in Ro/La autoantibody subsets. Anti-Ro60 were purified by elution from native Ro60-coated ELISA plates and subjected to combined de novo amino acid sequencing and database matching. Monospecific anti-Ro60 Igs comprised dominant public and minor private sets of IgG1 kappa and lambda restricted heavy and light chains. Specific IgV amino acid substitutions stratified anti-Ro60 from anti-Ro60/La responses, providing a molecular fingerprint of Ro60/La determinant spreading and suggesting that different forms of Ro60 antigen drive these responses. Sequencing of linked anti-Ro52 proteomes from individual patients and comparison with their anti-Ro60 partners revealed sharing of a dominant IGHV3-23/IGKV3-20 paired clonotype but with divergent IgV mutational signatures. In summary, anti-Ro60 IgV peptide mapping provides insights into Ro/La autoantibody diversification and reveals serum-based molecular markers of humoral Ro60 autoimmunity
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Autoantibody
|
| 650 |
|
4 |
|a Mass spectrometry sequencing
|
| 650 |
|
4 |
|a Ro60
|
| 650 |
|
4 |
|a Sjögren's syndrome
|
| 650 |
|
7 |
|a Autoantibodies
|2 NLM
|
| 650 |
|
7 |
|a Autoantigens
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin G
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin Variable Region
|2 NLM
|
| 650 |
|
7 |
|a Proteome
|2 NLM
|
| 650 |
|
7 |
|a RNA, Small Cytoplasmic
|2 NLM
|
| 650 |
|
7 |
|a RO60 protein, human
|2 NLM
|
| 650 |
|
7 |
|a Ribonucleoproteins
|2 NLM
|
| 700 |
1 |
|
|a Al Kindi, Mahmood A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Colella, Alex D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dykes, Lukah
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jackson, Michael W
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chataway, Tim K
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Reed, Joanne H
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gordon, Tom P
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 173(2016) vom: 14. Dez., Seite 57-63
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
|
| 773 |
1 |
8 |
|g volume:173
|g year:2016
|g day:14
|g month:12
|g pages:57-63
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.clim.2016.09.001
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_11
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 173
|j 2016
|b 14
|c 12
|h 57-63
|