Novel allelic variants in ACD6 cause hybrid necrosis in local collection of Arabidopsis thaliana

Novel allelic variants in ACD6 cause hybrid necrosis in local collection of Arabidopsis thaliana

© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1990. - 213(2017), 2 vom: 15. Jan., Seite 900-915
1. Verfasser: Świadek, Magdalena (VerfasserIn)
Weitere Verfasser: Proost, Sebastian, Sieh, Daniela, Yu, Jing, Todesco, Marco, Jorzig, Christian, Rodriguez Cubillos, Andrés Eduardo, Plötner, Björn, Nikoloski, Zoran, Chae, Eunyoung, Giavalisco, Patrick, Fischer, Axel, Schröder, Florian, Kim, Sang-Tae, Weigel, Detlef, Laitinen, Roosa A E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis thaliana ACCELERATED CELL DEATH 6 (ACD6) autoimmunity hybrid necrosis trade-off ACD6 protein, Arabidopsis Ankyrins Arabidopsis Proteins
Beschreibung
Zusammenfassung:© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
Hybrid necrosis is a common type of hybrid incompatibility in plants. This phenomenon is caused by deleterious epistatic interactions, resulting in spontaneous activation of plant defenses associated with leaf necrosis, stunted growth and reduced fertility in hybrids. Specific combinations of alleles of ACCELERATED CELL DEATH 6 (ACD6) have been shown to be a common cause of hybrid necrosis in Arabidopsis thaliana. Increased ACD6 activity confers broad-spectrum resistance against biotrophic pathogens but reduces biomass production. We generated 996 crosses among individuals derived from a single collection area around Tübingen (Germany) and screened them for hybrid necrosis. Necrotic hybrids were further investigated by genetic linkage, amiRNA silencing, genomic complementation and metabolic profiling. Restriction site associated DNA (RAD)-sequencing was used to understand genetic diversity in the collection sites containing necrosis-inducing alleles. Novel combinations of ACD6 alleles found in neighbouring stands were found to activate the A. thaliana immune system. In contrast to what we observed in controlled conditions, necrotic hybrids did not show reduced fitness in the field. Metabolic profiling revealed changes associated with the activation of the immune system in ACD6-dependent hybrid necrosis. This study expands our current understanding of the active role of ACD6 in mediating trade-offs between defense responses and growth in A.  thaliana
Beschreibung:Date Completed 09.02.2018
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.14155