The poor lonesome A subgenome of Brassica napus var. Darmor (AACC) may not survive without its mate

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 213(2017), 4 vom: 28. März, Seite 1886-1897
1. Verfasser:	Pelé, Alexandre (VerfasserIn)
Weitere Verfasser:	Trotoux, Gwenn, Eber, Frédérique, Lodé, Maryse, Gilet, Marie, Deniot, Gwenaelle, Falentin, Cyril, Nègre, Sylvie, Morice, Jérôme, Rousseau-Gueutin, Mathieu, Chèvre, Anne-Marie
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article allopolyploidy evolution oilseed rape structural rearrangements subgenome extraction

Beschreibung
Zusammenfassung:	© 2016 The INRA New Phytologist © 2016 New Phytologist Trust. Constitutive genomes of allopolyploid species evolve throughout their life span. However, the consequences of long-term alterations on the interdependency between each original genome have not been established. Here, we attempted an approach corresponding to subgenome extraction from a previously sequenced natural allotetraploid, offering a unique opportunity to evaluate plant viability and structural evolution of one of its diploid components. We employed two different strategies to extract the diploid AA component of the Brassica napus variety 'Darmor' (AACC, 2n = 4x = 38) and we assessed the genomic structure of the latest AA plants obtained (after four to five rounds of selection), using a 60K single nucleotide polymorphism Illumina array. Only one strategy was successful and the diploid AA plants that were structurally characterized presented a lower proportion of the B. napus A subgenome extracted than expected. In addition, our analyses revealed that some genes lost in a polyploid context appeared to be compensated for plant survival, either by conservation of genomic regions from B. rapa, used in the initial cross, or by some introgressions from the B. napus C subgenome. We conclude that as little as c. 7500 yr of coevolution could lead to subgenome interdependency in the allotetraploid B. napus as a result of structural modifications
Beschreibung:	Date Completed 22.02.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14147