Genome-wide identification and characterization of TCP genes involved in ovule development of Phalaenopsis equestris

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 17 vom: 01. Sept., Seite 5051-66
1. Verfasser: Lin, Yu-Fu (VerfasserIn)
Weitere Verfasser: Chen, You-Yi, Hsiao, Yu-Yun, Shen, Ching-Yu, Hsu, Jui-Ling, Yeh, Chuan-Ming, Mitsuda, Nobutaka, Ohme-Takagi, Masaru, Liu, Zhong-Jian, Tsai, Wen-Chieh
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cell proliferation Phalaenopsis equestris TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins. functional characterization genome-wide identification ovule development Transcription Factors
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520 |a TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins are plant-specific transcription factors known to have a role in multiple aspects of plant growth and development at the cellular, organ and tissue levels. However, there has been no related study of TCPs in orchids. Here we identified 23 TCP genes from the genome sequence of Phalaenopsis equestris Phylogenetic analysis distinguished two homology classes of PeTCP transcription factor families: classes I and II. Class II was further divided into two subclasses, CIN and CYC/TB1. Spatial and temporal expression analysis showed that PePCF10 was predominantly expressed in ovules at early developmental stages and PeCIN8 had high expression at late developmental stages in ovules, with overlapping expression at day 16 after pollination. Subcellular localization and protein-protein interaction analyses revealed that PePCF10 and PeCIN8 could form homodimers and localize in the nucleus. However, PePCF10 and PeCIN8 could not form heterodimers. In transgenic Arabidopsis thaliana plants (overexpression and SRDX, a super repression motif derived from the EAR-motif of the repression domain of tobacco ETHYLENE-RESPONSIVE ELEMENT-BINDING FACTOR 3 and SUPERMAN, dominantly repressed), the two genes helped regulate cell proliferation. Together, these results suggest that PePCF10 and PeCIN8 play important roles in orchid ovule development by modulating cell division 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Cell proliferation 
650 4 |a Phalaenopsis equestris 
650 4 |a TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins. 
650 4 |a functional characterization 
650 4 |a genome-wide identification 
650 4 |a ovule development 
650 7 |a Transcription Factors  |2 NLM 
700 1 |a Chen, You-Yi  |e verfasserin  |4 aut 
700 1 |a Hsiao, Yu-Yun  |e verfasserin  |4 aut 
700 1 |a Shen, Ching-Yu  |e verfasserin  |4 aut 
700 1 |a Hsu, Jui-Ling  |e verfasserin  |4 aut 
700 1 |a Yeh, Chuan-Ming  |e verfasserin  |4 aut 
700 1 |a Mitsuda, Nobutaka  |e verfasserin  |4 aut 
700 1 |a Ohme-Takagi, Masaru  |e verfasserin  |4 aut 
700 1 |a Liu, Zhong-Jian  |e verfasserin  |4 aut 
700 1 |a Tsai, Wen-Chieh  |e verfasserin  |4 aut 
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