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231224s2017 xx |||||o 00| ||eng c |
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7 |
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|a 10.1111/jfd.12537
|2 doi
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5 |
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|a pubmed25n0877.xml
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|a (NLM)27523084
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jin, J W
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection
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| 264 |
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 21.06.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a GENBANK: HQ620543, HQ620542, HM453864, HM453866, KF693765, KF693766, FJ917539, KF693768
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|a Citation Status MEDLINE
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|a © 2016 John Wiley & Sons Ltd.
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|a As suggested by the Office International des Epizooties (OIE), fishes belonging to the genus Oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream (Pagrus major). To assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including IL-8 and COX-2, and the antiviral factor like Mx from red sea bream for the first time and performed phylogenetic and structural analysis. Analysed expression levels of IL-1β, IL-8 and COX-2 and the antiviral factor like Mx genes performed with in vivo challenge experiment showed no difference in inflammatory gene expression or respiratory burst activity between red sea bream and rock bream (Oplegnathus fasciatus). However, the Mx gene expression levels in red sea bream were markedly higher than those in rock bream, suggesting the importance of type I interferon (IFN)-induced proteins, particularly Mx, during megalocytivirus infection, rather than inflammation-related genes. The in vitro challenge experiments using embryonic primary cultures derived from both fish species showed no difference in cytopathic effects (CPE), viral replication profiles, and inflammatory and Mx gene expression pattern between the two fish species
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4 |
|a Journal Article
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4 |
|a innate immune genes
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| 650 |
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4 |
|a megalocytivirus
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| 650 |
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4 |
|a red sea bream
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| 650 |
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4 |
|a rock bream
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| 650 |
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4 |
|a susceptibility
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| 650 |
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|a Fish Proteins
|2 NLM
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| 700 |
1 |
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|a Kim, Y C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hong, S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, M S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jeong, J B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jeong, H D
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 40(2017), 4 vom: 25. Apr., Seite 583-595
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
8 |
|g volume:40
|g year:2017
|g number:4
|g day:25
|g month:04
|g pages:583-595
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/jfd.12537
|3 Volltext
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