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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2016.07.012
|2 doi
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|a pubmed24n0876.xml
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|a (DE-627)NLM262819023
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|a (NLM)27459340
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|a (PII)S0981-9428(16)30279-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhang, Xue
|e verfasserin
|4 aut
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|a Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.04.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
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|a L-Ascorbic acid (AsA, ascorbate) is one of the most abundant natural antioxidants, and it is an important factor in the nutritional quality of cucumber. In this work, key enzymes involved in the ascorbic acid biosynthesis and recycling pathway in cucumber seedlings under nitrogen deficiency were investigated at the levels of transcription and enzyme activity. The activities of myo-inositol oxygenase (MIOX) and transcript levels of MIOXs increased dramatically, while the activities of ascorbate oxidase (AO) and glutathione reductase (GR) and transcript levels of AOs and GR2 decreased significantly in N-limited leaves, as did the ascorbate concentration, in nitrogen-deficient cucumber seedlings. The activities of other enzymes and transcript levels of other genes involved in the ascorbate recycling pathway and ascorbate synthesis pathways decreased or remained unchanged under nitrogen deficiency. These results indicate that nitrogen deficiency induced genes involved in the ascorbate-glutathione recycling and myo-inositol pathway in cucumber leaves. Thus, the AO, GR and MIOX involved in the pathways might play roles in AsA accumulation
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|a Journal Article
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|a Ascorbate biosynthesis pathways
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|a Ascorbate recycling pathway
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|a Cucumber
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|a L-ascorbic acid
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|a Nitrogen deficiency
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|a Ascorbate Oxidase
|2 NLM
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|a EC 1.10.3.3
|2 NLM
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|a Glutathione Reductase
|2 NLM
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|a EC 1.8.1.7
|2 NLM
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 650 |
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|a Ascorbic Acid
|2 NLM
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| 650 |
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|a PQ6CK8PD0R
|2 NLM
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| 700 |
1 |
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|a Yu, Hong Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xiao Meng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Xue Yong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Wen Chao
|e verfasserin
|4 aut
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| 700 |
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|a Li, Qiang
|e verfasserin
|4 aut
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| 700 |
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|a Jiang, Wei Jie
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 108(2016) vom: 02. Nov., Seite 222-230
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:108
|g year:2016
|g day:02
|g month:11
|g pages:222-230
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|u http://dx.doi.org/10.1016/j.plaphy.2016.07.012
|3 Volltext
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|d 108
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|h 222-230
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