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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw290
|2 doi
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|a pubmed24n1423.xml
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|e rakwb
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|a eng
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|a Fukudome, Mitsutaka
|e verfasserin
|4 aut
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|a Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 07.11.2017
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|a Date Revised 30.05.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5'-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels
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|a Journal Article
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|a Hemoglobin
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|a Lotus japonicus
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|a Mesorhizobium loti
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|a nitric oxide
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|a nitrogen fixation
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|a nodulation mutants
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|a symbiosis.
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|a Hemoglobins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Nitric Oxide
|2 NLM
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|a 31C4KY9ESH
|2 NLM
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| 700 |
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|a Calvo-Begueria, Laura
|e verfasserin
|4 aut
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|a Kado, Tomohiro
|e verfasserin
|4 aut
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|a Osuki, Ken-Ichi
|e verfasserin
|4 aut
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|a Rubio, Maria Carmen
|e verfasserin
|4 aut
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|a Murakami, Ei-Ichi
|e verfasserin
|4 aut
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|a Nagata, Maki
|e verfasserin
|4 aut
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|a Kucho, Ken-Ichi
|e verfasserin
|4 aut
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|a Sandal, Niels
|e verfasserin
|4 aut
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|a Stougaard, Jens
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|a Becana, Manuel
|e verfasserin
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|a Uchiumi, Toshiki
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 17 vom: 16. Sept., Seite 5275-83
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:67
|g year:2016
|g number:17
|g day:16
|g month:09
|g pages:5275-83
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|u http://dx.doi.org/10.1093/jxb/erw290
|3 Volltext
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