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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b01679
|2 doi
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|a pubmed24n0874.xml
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|a eng
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|a Micheletto, Yasmine Miguel Serafini
|e verfasserin
|4 aut
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| 245 |
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|a Electroformation of Giant Unilamellar Vesicles
|b Investigating Vesicle Fusion versus Bulge Merging
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.06.2018
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|a Date Revised 01.06.2018
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Partially ordered stacks of phospholipid bilayers on a flat substrate can be obtained by the evaporation of a spread droplet of phospholipid-in-chloroform solution. When exposed to an aqueous buffer, numerous micrometric buds populate the bilayers, grow in size over minutes, and eventually detach, forming the so-called liposomes or vesicles. While observation of vesicle growth from a hydrated lipid film under an optical microscope suggests numerous events of vesicle fusion, there is little experimental evidence for discriminating between merging of connected buds, i.e., a shape transformation that does not imply bilayer fusion and real membrane fusion. Here, we use electroformation to grow giant unilamellar vesicles (GUVs) from a stack of lipids in a buffer containing either (i) nanometric liposomes or (ii) previously prepared GUVs. By combining different fluorescent labels of the lipids in the substrate and in the solution, and by performing a fluorescence analysis of the resulting GUVs, we clearly demonstrate that merging of bulges is the essential pathway for vesicle growth in electroformation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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1 |
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|a Marques, Carlos M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Silveira, Nádya Pesce da
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schroder, André P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 32 vom: 16. Aug., Seite 8123-30
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:32
|g year:2016
|g number:32
|g day:16
|g month:08
|g pages:8123-30
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|u http://dx.doi.org/10.1021/acs.langmuir.6b01679
|3 Volltext
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