A phased strategy to differentiate human CD14+monocytes into classically and alternatively activated macrophages and dendritic cells
There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells (PBMCs) into the M1 or M2 macrophage cell types. Each cell type is then verified using flow cytometric analysis of cell-surface markers. Human CD14(+) monocytes h...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 61(2016), 1 vom: 07., Seite 33-41
|
|---|
| 1. Verfasser: |
Zarif, Jelani C
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Hernandez, James R,
Verdone, James E,
Campbell, Scott P,
Drake, Charles G,
Pienta, Kenneth J |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2016
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
M1 macrophages
M2 macrophages
cytokine
macrophage polarization
monocyte differentiation
monocyte-derived dendritic cells
multi-nucleated giant cells
mehr...
peripheral blood mononuclear cells
phased strategy
IL6 protein, human
Interleukin-6
Lipopolysaccharide Receptors |
|---|