Two tomato GDP-D-mannose epimerase isoforms involved in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development

Two tomato GDP-D-mannose epimerase isoforms involved in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 15 vom: 04. Aug., Seite 4767-77
1. Verfasser: Mounet-Gilbert, Louise (VerfasserIn)
Weitere Verfasser: Dumont, Marie, Ferrand, Carine, Bournonville, Céline, Monier, Antoine, Jorly, Joana, Lemaire-Chamley, Martine, Mori, Kentaro, Atienza, Isabelle, Hernould, Michel, Stevens, Rebecca, Lehner, Arnaud, Mollet, Jean Claude, Rothan, Christophe, Lerouge, Patrice, Baldet, Pierre
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ascorbate GDP-D-mannose epimerase Solanum lycopersicum cell wall growth rhamnogalacturonan-II tomato fruits. Isoenzymes mehr... Carbohydrate Epimerases EC 5.1.3.- GDPmannose 3,5-epimerase EC 5.1.3.18 Ascorbic Acid PQ6CK8PD0R
Beschreibung
Zusammenfassung:© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective role in ascorbate and cell wall biosynthesis of the two SlGME genes present in tomato by targeting each of them through an RNAi-silencing approach. Taken individually SlGME1 and SlGME2 allowed normal ascorbate accumulation in the leaf and fruits, thus suggesting the same function regarding ascorbate. However, SlGME1 and SlGME2 were shown to play distinct roles in cell wall biosynthesis, depending on the tissue considered. The RNAi-SlGME1 plants harbored small and poorly seeded fruits resulting from alterations of pollen development and of pollination process. In contrast, the RNAi-SlGME2 plants exhibited vegetative growth delay while fruits remained unaffected. Analysis of SlGME1- and SlGME2-silenced seeds and seedlings further showed that the dimerization state of pectin rhamnogalacturonan-II (RG-II) was altered only in the RNAi-SlGME2 lines. Taken together with the preferential expression of each SlGME gene in different tomato tissues, these results suggest sub-functionalization of SlGME1 and SlGME2 and their specialization for cell wall biosynthesis in specific tomato tissues
Beschreibung:Date Completed 06.11.2017
Date Revised 29.05.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/erw260