The barley (Hordeum vulgare) cellulose synthase-like D2 gene (HvCslD2) mediates penetration resistance to host-adapted and nonhost isolates of the powdery mildew fungus

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 212(2016), 2 vom: 21. Okt., Seite 421-33
1. Verfasser:	Douchkov, Dimitar (VerfasserIn)
Weitere Verfasser:	Lueck, Stefanie, Hensel, Goetz, Kumlehn, Jochen, Rajaraman, Jeyaraman, Johrde, Annika, Doblin, Monika S, Beahan, Cherie T, Kopischke, Michaela, Fuchs, René, Lipka, Volker, Niks, Rients E, Bulone, Vincent, Chowdhury, Jamil, Little, Alan, Burton, Rachel A, Bacic, Antony, Fincher, Geoffrey B, Schweizer, Patrick
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Blumeria graminis CSL RNAi cell wall cellulose synthase-like transgenic barley plants Plant Proteins Polysaccharides Glucosyltransferases mehr... EC 2.4.1.- cellulose synthase

Beschreibung
Zusammenfassung:	© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust. Cell walls and cellular turgor pressure shape and suspend the bodies of all vascular plants. In response to attack by fungal and oomycete pathogens, which usually breach their host's cell walls by mechanical force or by secreting lytic enzymes, plants often form local cell wall appositions (papillae) as an important first line of defence. The involvement of cell wall biosynthetic enzymes in the formation of these papillae is still poorly understood, especially in cereal crops. To investigate the role in plant defence of a candidate gene from barley (Hordeum vulgare) encoding cellulose synthase-like D2 (HvCslD2), we generated transgenic barley plants in which HvCslD2 was silenced through RNA interference (RNAi). The transgenic plants showed no growth defects but their papillae were more successfully penetrated by host-adapted, virulent as well as avirulent nonhost isolates of the powdery mildew fungus Blumeria graminis. Papilla penetration was associated with lower contents of cellulose in epidermal cell walls and increased digestion by fungal cell wall degrading enzymes. The results suggest that HvCslD2-mediated cell wall changes in the epidermal layer represent an important defence reaction both for nonhost and for quantitative host resistance against nonadapted wheat and host-adapted barley powdery mildew pathogens, respectively
Beschreibung:	Date Completed 05.02.2018 Date Revised 30.09.2020 published: Print-Electronic GENBANK: KP122993 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14065