Physiological characterization and true-to-typeness evaluation of in vitro and ex vitro seedlings of Pinus elliottii A contribution to breeding programs

Physiological characterization and true-to-typeness evaluation of in vitro and ex vitro seedlings of Pinus elliottii : A contribution to breeding programs

Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 107(2016) vom: 03. Okt., Seite 222-227
1. Verfasser: Nunes, Sandra (VerfasserIn)
Weitere Verfasser: Santos, Conceição, Moutinho-Pereira, José, Correia, Carlos, Oliveira, Helena, Ferreira de Oliveira, José Miguel, Pereira, Vanessa Tolentino, Almeida, Tânia, Marum, Liliana, Dias, Maria Celeste
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Genetic stability Micropropagation Photosynthesis Slash pine Carbohydrates Gases Water 059QF0KO0R Chlorophyll mehr... 1406-65-1 Chlorophyll A YF5Q9EJC8Y
Beschreibung
Zusammenfassung:Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Pinus elliottii var. elliottii is a pine species with enormous economic value particularly for timber and resin industries, and is subject of high pressure for genetic improvement and cloning elite genotypes. We have recently developed a robust micropropagation protocol for this species. Plantlets performance needs to be evaluated to validate this protocol for further mass propagation. Micropropagated plantlets and seed-derived plants with similar age and shoot length were compared regarding photosynthesis, carbohydrates and pigments content, water status, DNA content and cell cycle dynamics. Micropropagated plantlets had an overall physiological performance similar to seed-derived plants. In particular, except for the transpiration rate (E), CO2 assimilation rate (A) and total soluble sugars (TSS) content, no major differences between plantlets and seedlings in terms of relative water content (RWC), chlorophyll a fluorescence and pigments content were found. Genetic fidelity analyses support that the micropropagation protocol neither induce DNA content changes nor alterations in cell cycle dynamics
Beschreibung:Date Completed 27.03.2017
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2016.05.039