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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b01487
|2 doi
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|a pubmed24n0871.xml
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|a eng
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| 100 |
1 |
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|a Draffehn, Sören
|e verfasserin
|4 aut
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| 245 |
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|a Insight into the Modification of Polymeric Micellar and Liposomal Nanocarriers by Fluorescein-Labeled Lipids and Uptake-Mediating Lipopeptides
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 05.06.2018
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|a Date Revised 18.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Encapsulation of diagnostic and therapeutic compounds in transporters improves their delivery to the point of need. An even more efficient treatment of diseases can be achieved using carriers with targeting or protecting moieties. In the present work, we investigated micellar and liposomal nanocarriers modified with fluorescein, peptides, and polymers that are covalently bound to fatty acids or phospholipids to ensure a self-driven incorporation into the micelles or liposomes. First, we characterized the photophysics of the fluorescent probes in the absence and in the presence of nanocarriers. Changes in the fluorescence decay time, quantum yield, and intensity of a fluorescein-labeled fatty acid (fluorescein-labeled palmitic acid [fPA]) and a fluorescein-labeled lipopeptide (P2fA2) were found. By exploiting these changes, we investigated a lipopeptide (P2A2 as an uptake-mediating unit) in combination with different nanocarriers (micelles and liposomes) and determined the corresponding association constant Kass values, which were found to be very high. In addition, the mobility of fPA was exploited using fluorescence correlation spectroscopy (FCS) and fluorescence depolarization (FD) experiments to characterize the nanocarriers. Cellular uptake experiments with mouse brain endothelial cells provided information on the uptake behavior of liposomes modified by uptake-mediating P2A2 and revealed differences in the uptake behavior between pH-sensitive and pH-insensitive liposomes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lipopeptides
|2 NLM
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|a Liposomes
|2 NLM
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|a Palmitic Acid
|2 NLM
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|a 2V16EO95H1
|2 NLM
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|a Fluorescein
|2 NLM
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|a TPY09G7XIR
|2 NLM
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1 |
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|a Eichhorst, Jenny
|e verfasserin
|4 aut
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|a Wiesner, Burkhard
|e verfasserin
|4 aut
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| 700 |
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|a Kumke, Michael U
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 27 vom: 12. Juli, Seite 6928-39
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:32
|g year:2016
|g number:27
|g day:12
|g month:07
|g pages:6928-39
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|u http://dx.doi.org/10.1021/acs.langmuir.6b01487
|3 Volltext
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