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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b01377
|2 doi
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|a pubmed24n0870.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Biswas, Kabir H
|e verfasserin
|4 aut
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| 245 |
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|a A Microbead Supported Membrane-Based Fluorescence Imaging Assay Reveals Intermembrane Receptor-Ligand Complex Dimension with Nanometer Precision
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 05.06.2018
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|a Date Revised 04.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Receptor-ligand complexes spanning a cell-cell interface inevitably establish a preferred intermembrane spacing based on the molecular dimensions and orientation of the complexes. This couples molecular binding events to membrane mechanics and large-scale spatial organization of receptors on the cell surface. Here, we describe a straightforward, epi-fluorescence-based method to precisely determine intermembrane receptor-ligand dimension at adhesions established by receptor-ligand binding between apposed membranes in vitro. Adhesions were reconstituted between planar and silica microbead supported membranes via specific interaction between cognate receptor/ligand pairs (EphA2/EphrinA1 and E-cadherin/anti-E-cadherin antibody). Epi-fluorescence imaging of the ligand enrichment zone in the supported membrane beneath the adhering microbead, combined with a simple geometrical interpretation, proves sufficient to estimate intermembrane receptor-ligand dimension with better than 1 nm precision. An advantage of this assay is that no specialized equipment or imaging methods are required
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antibodies
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|a Antigens, CD
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|a CDH1 protein, human
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|a Cadherins
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|a EPHA2 protein, human
|2 NLM
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|a Ephrin-A1
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|a Ephrin-A2
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|a Silicon Dioxide
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|a Groves, Jay T
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 26 vom: 05. Juli, Seite 6775-80
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:32
|g year:2016
|g number:26
|g day:05
|g month:07
|g pages:6775-80
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|u http://dx.doi.org/10.1021/acs.langmuir.6b01377
|3 Volltext
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