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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2016.05.028
|2 doi
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|a pubmed24n0870.xml
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|a (DE-627)NLM261058940
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|a (NLM)27262101
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|a (PII)S0981-9428(16)30198-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Sin, I N
|e verfasserin
|4 aut
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|a Analysis of the carbohydrate-binding-module from Fragaria x ananassa α-L-arabinofuranosidase 1
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.03.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
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|a α-L-arabinofuranosidases (EC 3.2.1.55) are enzymes involved in the catabolism of several cell-wall polysaccharides such as pectins and hemicelluloses, catalyzing the hydrolysis of terminal non-reducing α-L-arabinofuranosil residues. Bioinformatic analysis of the aminoacidic sequences of Fragaria x ananassa α-L-arabinofuranosidases predict a putative carbohydrate-binding-module of the family CBM_4_9, associated to a wide range of carbohydrate affinities. In this study, we report the characterization of the binding affinity profile to different cell wall polysaccharides of the putative CBM of α-L-arabinofuranosidase 1 from Fragaria x ananassa (CBM-FaARA1). The sequence encoding for the putative CBM was cloned and expressed in Escherichia coli, and the resultant recombinant protein was purified from inclusion bodies by a Nickel affinity chromatography under denaturing conditions. The refolded recombinant protein was then subjected to binding assays and affinity gel electrophoresis, which indicated its ability to bind cellulose and also high affinity for homogalacturonans
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|a Journal Article
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|a Carbohydrate binding module (CBM)
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|a Cell wall
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|a Heterologous expression
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|a Homogalacturonans
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|a Strawberry
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|a α-L-arabinofuranosidas
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|a Receptors, Cell Surface
|2 NLM
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|a Recombinant Proteins
|2 NLM
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|a saccharide-binding proteins
|2 NLM
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|a Glycoside Hydrolases
|2 NLM
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|a EC 3.2.1.-
|2 NLM
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| 650 |
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|a alpha-N-arabinofuranosidase
|2 NLM
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| 650 |
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|a EC 3.2.1.55
|2 NLM
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| 700 |
1 |
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|a Perini, M A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez, G A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Civello, P M
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 107(2016) vom: 01. Okt., Seite 96-103
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:107
|g year:2016
|g day:01
|g month:10
|g pages:96-103
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|u http://dx.doi.org/10.1016/j.plaphy.2016.05.028
|3 Volltext
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|d 107
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|b 01
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|h 96-103
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