The RICE MINUTE-LIKE1 (RML1) gene, encoding a ribosomal large subunit protein L3B, regulates leaf morphology and plant architecture in rice

The RICE MINUTE-LIKE1 (RML1) gene, encoding a ribosomal large subunit protein L3B, regulates leaf morphology and plant architecture in rice

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 11 vom: 15. Mai, Seite 3457-69
1. Verfasser: Zheng, Ming (VerfasserIn)
Weitere Verfasser: Wang, Yihua, Liu, Xi, Sun, Juan, Wang, Yunlong, Xu, Yang, Lv, Jia, Long, Wuhua, Zhu, Xiaopin, Guo, Xiuping, Jiang, Ling, Wang, Chunming, Wan, Jianmin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Minute mutants Oryza sativa plant development plant growth ribosomal proteins ribosome biogenesis rice vascular patterning. mehr... Plant Proteins Ribosomal Proteins
Beschreibung
Zusammenfassung:© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Mutations of ribosomal proteins (RPs) are known to cause developmental abnormalities in yeast, mammals, and dicotyledonous plants; however, their effects have not been studied in rice. Here, we identifiy a ribosomal biogenesis mutant, rice minute-like1 (rml1) that displays a minute phenotype as evidenced by retarded growth and defects in the vascular system. We determine that RML1 encodes a ribosome large subunit protein 3B (RPL3B) in rice by means of map-based cloning and genetic complementation. RPL3B is abundantly expressed in all the tissues, whereas RPL3A, another RPL3 gene family member, is expressed at low levels. Notably, the expression level of RPL3A in the rml1 mutant is similar to that in the wild-type, suggesting that RPL3A provides no functional compensation for RPL3B in rml1 plants. Ribosomal profiles show that mutation of RPL3B leads to a significant reduction in free 60S ribosomal subunits and polysomes, indicating a ribosomal insufficiency in the rml1 mutant. Our results demonstrate that the ribosomal protein gene RPL3B is required for maintaining normal leaf morphology and plant architecture in rice through its regulation of ribosome biogenesis
Beschreibung:Date Completed 20.12.2017
Date Revised 09.04.2022
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/erw167