Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis

Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 106(2016) vom: 16. Sept., Seite 288-94
1. Verfasser: Gou, Jun-Bo (VerfasserIn)
Weitere Verfasser: Li, Zhen-Qiu, Li, Chang-Fu, Chen, Fang-Fang, Lv, Shi-You, Zhang, Yan-Sheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article 10-Epi-junenol synthase Eudesmanolide Inula hupehensis DNA, Complementary Plant Proteins Polyisoprenyl Phosphates RNA, Messenger Sesquiterpenes eudesmanolide mehr... farnesyl pyrophosphate 79W6B01D07 Alkyl and Aryl Transferases EC 2.5.- terpene synthase EC 2.5.1.-
Beschreibung
Zusammenfassung:Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Junenol based-eudesmanolides have been detected in many compositae plant species and were reported to exhibit various pharmacological activities. So far, the gene encoding junenol synthase has never been isolated. Here we report the molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis (designated IhsTPS1). IhsTPS1 converts the substrate farnesyl diphosphate into multiple sesquiterpenes with the product 10-epi-junenol being predominant. The transcript levels of IhsTPS1 correlate well with the accumulation pattern of 10-epi-junenol in I. hupehensis organs, supporting its biochemical roles in vivo
Beschreibung:Date Completed 27.03.2017
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2016.05.023