Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation

OBJECTIVE: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa c...

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Veröffentlicht in:Asian-Australasian journal of animal sciences. - 1998. - 30(2017), 1 vom: 07. Jan., Seite 20-33
1. Verfasser: Wu, Yangsheng (VerfasserIn)
Weitere Verfasser: Lin, Jiapeng, Li, Xiaolin, Han, Bing, Wang, Liqin, Liu, Mingjun, Huang, Juncheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Asian-Australasian journal of animal sciences
Schlagworte:Journal Article Granulosa Cells Lamb RNA Seq Sheep Superstimulation
Beschreibung
Zusammenfassung:OBJECTIVE: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role
METHODS: In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep
RESULTS: There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly down-regulated in the superstimulated follicles. Ingenuity pathway and gene ontology analyses revealed that processes of axonal guidance, cell proliferation and DNA replication were expressed at higher levels in the prepubertal follicles. Epidermal growth factor, T-Box protein 2 and beta-estradiol upstream regulator were predicted to be active in prepubertal follicles. By comparison, tumor protein P53 and let-7 were most active in adult follicles
CONCLUSION: These results may contribute to a better understanding of the mechanisms governing the development of granulosa cells in the growing follicle in prepubertal sheep
Beschreibung:Date Revised 30.09.2020
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1011-2367
DOI:10.5713/ajas.15.0999